Question

write instructions how to pipette 5 microliters of fluid AND draw a diagram that shows how to complete the serial dilutions to conduct. Diagram of a Serial Dilution (use P-1000 set to 5,0,0)

Answer 1

The liquid of 5µl accurately be pippeted using a micropipette in the range of 0-10 µl or 5-20 µl.

Making serial dilutions

            A series of simple dilutions amplify the small initial quantity of material. The dilution material (solute) for each step comes from the diluted material of the previous dilution step. The first step is to take a known volume of stock and dilute it with known volume of solvent preferably distilled water.

Example – If 1 ml stock solution is added to 9 ml of water, it produces 10ml of the dilute solution (A) which have the concentration of 1ml of extract /10ml. The amount of stock in each ml of the diluted solution is 0.1ml. If 1 ml of this stock (A) is taken and diluted by adding 9 ml of distilled water it gives concentration of 0.1 ml/10 ml (B). The amount of stock in diluted solution is 0.01 ml). Again if 1 ml of solution is taken from stock B and 9 ml distilled water is added it gives concentration of 0.01 ml of extract /10 ml.