In animal tissue, the rate of conversion of pyruvate of acetyl-CoA is regulated by the ratio...

In animal tissue, the rate of conversion of pyruvate of acetyl-CoA is regulated by the ratio of active, phosphorylated pyruvate dehydrogenase to inactive, unphosphorylated pyruvate dehydrogenase (PDG) complex. Explain the impact of the following regulators on the activity of pyruvate dehydrogenase complex in animal tissue extracts when treated with:

- high concentrations of acetyl-CoA

Expert Solution

Answer . Effect of high concentrations of Acetyl Co A on PDH complex-

Pyruvate dehydrogenase complex is a multienzyme system, that contains 3 enzymes to fullfil one goal ( conversion of pyruvate into acetyl CoA).

These three enzymes are

E1- pyruvate dehydrogenase

E2- dihydrolipoyl trans acetylase

E3- dihydrolipoyl dehydrogenase.

Pyruvate dehydrogenase is active when it is a dephosphoenzyme and it is inactive when it is a phosphoenzyme . The attachment of phosphate group is done by PDH kinase enzyme. When Acetyl coA is in excess it activated the PDH kinase enzyme to form inactive phosphoenzyme a, and inhibits the activity of PDH complex.

For diagrammatic presentation kindly refer image.

gladiator answered 1 year ago

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