What does “semi-discontinuous” DNA replication mean? Explain in
words how the lagging DNA strand is replicated in bacteria. What
specific enzymatic activities does DNA polymerase I have? Name each
activity and explain its role in DNA replication. If DNA polymerase
I lacked its 3’-5’ exonuclease activity, what specific consequences
with respect to DNA replication would be observed? What
consequences would be observed if, instead of DNA pol I, DNA
polymerase III lacked its 3’-5’ exonuclease activity? Explain your
answers.
In
electrophoresis of suspect DNA in the crime scene, what does it
mean when when we say that DNA samples were fragmented? What would
your gel look like if the DNA was not fragmented? Explain using
scientific evidence (10 points)