In a PCR experiment, what does it mean when the positive control has a DNA band,...

In a PCR experiment, what does it mean when the positive control has a DNA band, but the experimental tube does not?

Solutions

Expert Solution

Positive control in PCR reaction is a control having all the PCR components and PCR condition best suited for that DNA sample. In contrast the negative DNA control also has all the PCR components and conditions but it does not have templet DNA. Hence it cannot give any band. Negative controls is used to check any contamination in DNA sample. If it is contaminated it can amplify and gives band.

On the other hand positive PCR control always gives band because it has all the PCR components and templets. If band is present in positive control and no band is present in our experimental tube indicates that something is wrong with our experiment. Some of the component of PCR are not added or added in Lower concentration (dNTPS, MgCl2, PCR buffer, DNA templet, primer or other components). Or the PCR condition is not maintained as per the requirement.

gladiator answered 1 year ago

Next > < Previous

Related Solutions

When setting up a PCR experiment, you must prepare a positive control sample. What would the...

When setting up a PCR experiment, you must prepare a positive control sample. What would the positive control help you discover? A) You forgot to add Loading Dye to your samples B) The DNA ladder is denatured C) Your thermocycler is broken D) Your reagents are contaminated with foreign DNA

What is the purpose of a a “No DNA” control reaction? What does it mean if...

What is the purpose of a a “No DNA” control reaction? What does it mean if the gel lane has bands?

What does ‘antiparallel’ mean when referring to DNA?

what does the polymerase chain reaction (PCR) accomplish? 1) PCR converts RNA to DNA 2) PCR...

what does the polymerase chain reaction (PCR) accomplish? 1) PCR converts RNA to DNA 2) PCR corrects mutation in a gene 3) PCR makes many copies of DNA segment 4) PCR joins short segments of DNA to make one long segment how does the ribosome select the next amino acid to add to a growing polypeptide chain? 1) the ribosome selects the amino acid that can form hydrogen bonds with the mRNA 2) the ribosome selects a tRNA that base-pairs...

What does it mean when it is said that DNA replication is ‘semiconservative’?

In electrophoresis of suspect DNA in the crime scene, what does it mean when when we...

In electrophoresis of suspect DNA in the crime scene, what does it mean when when we say that DNA samples were fragmented? What would your gel look like if the DNA was not fragmented? Explain using scientific evidence (10 points)

You Explain it: What is a residual? What does it mean when a residual is positive?...

You Explain it: What is a residual? What does it mean when a residual is positive? Explain the phrase outside the scope of the model. Why is it dangerous to make predictions outside the scope of the model? Explain what each point on the least-squares regression line represents.

What does it mean to linearize DNA?

what does it mean if DNA is coding

1.) What is the positive control in this experiment? Negative control? Why do you think this?...

1.) What is the positive control in this experiment? Negative control? Why do you think this? Tube # 3 M Na Pyruvate(Activator) 0.1 M MgSO4 (activator) 0.1 M NaF (inhibitor) 5.0% Glucose (Activator) Water Fill With Co2 Produced after 40 min 1 - - - - 7.5 mL Yeast .10 mm 2 - - - 2.5 mL 5.0 mL Yeast 2.5 mm 3 - 5.0 mL - 2.5 mL - Yeast 5.0 mm 4 - - 0.5 mL 2.5 mL...

Subjects