In: Biology
Answer these questions regarding DNA libraries:
In creating a genomic DNA library, what is the FIRST step?
a. |
Fragmenting the DNA into manageable pieces |
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b. |
Screening clones |
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c. |
Transforming recombinant DNA into bacteria |
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d. |
Ligating DNA into plasmids |
Sometimes artificial chromosomes (AC) constructed from bacterial (BACs) or yeast (YACs) DNA are used to harbor genomic DNA for libraries (e.g., the human genome project). Why are BACs/YACs often used instead of traditional plasmid DNA for the construction of genomic libraries?
a. |
genomic plasmid DNA cannot be efficiently ligated |
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b. |
Typical plasmid DNA vectors can only harbor DNA up to a certain size (~3,000 bp) |
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c. |
BACs/YACs are easier to store in DNA libraries |
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d. |
Larger genomic DNA fragments in BACS/YACS have the introns removed |
Which library would you screen if the goal was to identify the coding sequence for a protein?
a. |
genomic library |
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b. |
RNA library |
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c. |
cDNA library |
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d. |
protein domain library |
How does one find a book (gene/clone) of interest in a DNA library?
a. |
by doing PCR on the whole pool of DNA |
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b. |
by sequencing each clone in the library |
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c. |
by next-generation sequencing |
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d. |
by hybridizing a radiolabeled probe of the sequence to bacterial colonies |
1. a, Fragmenting the DNA into manageable pieces
2. b, Typical plasmid DNA vectors can only harbor DNA up to a certain size (~3,000 bp)