Answer these questions regarding DNA libraries: In creating a genomic DNA library, what is the FIRST...

Answer these questions regarding DNA libraries:

In creating a genomic DNA library, what is the FIRST step?

	a.	Fragmenting the DNA into manageable pieces
	b.	Screening clones
	c.	Transforming recombinant DNA into bacteria
	d.	Ligating DNA into plasmids

Sometimes artificial chromosomes (AC) constructed from bacterial (BACs) or yeast (YACs) DNA are used to harbor genomic DNA for libraries (e.g., the human genome project). Why are BACs/YACs often used instead of traditional plasmid DNA for the construction of genomic libraries?

	a.	genomic plasmid DNA cannot be efficiently ligated
	b.	Typical plasmid DNA vectors can only harbor DNA up to a certain size (~3,000 bp)
	c.	BACs/YACs are easier to store in DNA libraries
	d.	Larger genomic DNA fragments in BACS/YACS have the introns removed

Which library would you screen if the goal was to identify the coding sequence for a protein?

	a.	genomic library
	b.	RNA library
	c.	cDNA library
	d.	protein domain library

How does one find a book (gene/clone) of interest in a DNA library?

	a.	by doing PCR on the whole pool of DNA
	b.	by sequencing each clone in the library
	c.	by next-generation sequencing
	d.	by hybridizing a radiolabeled probe of the sequence to bacterial colonies

Solutions

Expert Solution

1. a, Fragmenting the DNA into manageable pieces

2. b, Typical plasmid DNA vectors can only harbor DNA up to a certain size (~3,000 bp)

gladiator answered 1 year ago

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