summarize the process by which the genomic library of P.fluorescens DNA was created. In fact, I...

summarize the process by which the genomic library of P.fluorescens DNA was created.

In fact, I don’t understand the question, please help me answer this question, if you would explain this question in a better way, that will be great!

Expert Solution

Pseudomonas fluorescens are highly diverse.Strain specific regions around the replication terminus suggest genome compartmentalization.The genome heterogenicity among the three strains is reminiscent of a species complex rather than a single species.The 42% of plant inducible genes were not shared by all strains reinforces this success specialised core functional genes.

Constructiuon of the genomic library involves creating many recombinanat DNA molecules.An organism's genomic DNA is extraxted and then digested with a restriction enzyme.The digested fragments are separated by gel electrophoresis.The separated fragments can then be exised and clones into the vector separately.However when a larger genome is digested with arestriction enzyme,there are too many fragments to exise individually.Then the vector containing the inserted fragments of genomic DNA can be then be introduced into a host organism.

gladiator answered 1 year ago

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