Question

1. Which of the following statement is FALSE?

   	A.	Gel electrophoresis is used to separate DNA according to their size
   	B.	Chloroform is used for DNA precipitation
   	C.	PCR amplifies DNA to millions of copies
   	D.	Introns code for non-coding sequence whereas exons code for coding sequences

2. Genotype frequency of the Alu insert in a population could be

   	A.	homozygotes (-/-) and homozygotes (+/+)
   	B.	heterozygous (+/-) and mongoloid population
   	C.	homozygotes (+/+) and asian population
   	D.	homozygotes (+/+) and mongoloid population

3. In a SEQUENTIAL order, what are the three steps in PCR?

   	A.	Denature DNA, Anneal Primers, Extend DNA
   	B.	Anneal Primers, Extend DNA, Denature DNA
   	C.	Denature DNA, Extend DNA, Anneal Primers
   	D.	Extend DNA, Anneal Primers, Denature DNA

Answer 1

Question Which of the following statement is FALSE?

	A.	Gel electrophoresis is used to separate DNA according to their size
	B.	Chloroform is used for DNA precipitation
	C.	PCR amplifies DNA to millions of copies
	D.	Introns code for non-coding sequence whereas exons code for coding sequences

Answer: Option B

Explanation: A. DNA seprated based based on the charge and size using Gel electrophoresis is correct option

B. Chloroform is used for DNA precipitation is wrong statement (Ethanol is used for DNA precipitation)

C. PCR amplifies DNA to millions of copies is correct

D. Exons - COding region of DNA and Introns are non-coding region

Question 2: In a SEQUENTIAL order, what are the three steps in PCR?

	A.	Denature DNA, Anneal Primers, Extend DNA
	B.	Anneal Primers, Extend DNA, Denature DNA
	C.	Denature DNA, Extend DNA, Anneal Primers
	D.	Extend DNA, Anneal Primers, Denature DNA

PCR follow the three steps

1) DNA denaturation

2) Primer annealing and

3) Extension