Compare and contrast protein (SDS-PAGE) and DNA (agarose) gel electrophoresis.

Expert Solution

Difference between Protein (SDS-PAGE) gel electrophoresis and DNA (agarose) gel electrophoresis is as follows:

	Protein (SDS-PAGE) gel electrophoresis	DNA (agarose) gel electrophoresis
Name of gel used	Sodium dodecyl sulfate (SDS) polyacrylamide gel slabs is used.	Agarose gel is used.
Sepration	It is used for sepration of protein separation (Neutral PAGE can be used for DNA separation)	It is used for sepration of DNA and Nucleic acids.
Principle	It is based on Mass as well as charge.	It is based on the principle of charge and size of nucleic acids.
Resolution	When the PAGE gel (neutral) when used for DNA can give better resolution than Agarose gel.	It gives low resolution for DNA than SDS-PAGE gel.
Denaturation	In this electrophoresis proteins denatures prior to separation.	In this electrophoresis both denaturating and non denaturating technicques used.
Gel running	Always vertically.	Horizontally or vertically.
Uses	It is used for sepration of proteins based on their mass.	It is used for macromolecules using an electric field.

gladiator answered 1 year ago

In agarose gel electrophoresis of a sample of DNA, A) small DNA fragments in the sample...

In agarose gel electrophoresis of a sample of DNA, A) small DNA fragments in the sample will move more rapidly through the gel than large DNA fragments. B) the DNA will move through the gel toward the positive electrode. C) the higher the concentration of agarose in the gel, the more rapidly will the DNA move through the gel. D) A and B E) all of the above

Compare and contrast native gel electrophoresis and denaturing gel electrophoresis. Which method would be used to...

Compare and contrast native gel electrophoresis and denaturing gel electrophoresis. Which method would be used to determine the molecular weight of a protein, why?

What are the components of agarose gel electrophoresis set up?

Linda has asked Brenn to prepare a 1% agarose gel for a gel electrophoresis analysis. But...

Linda has asked Brenn to prepare a 1% agarose gel for a gel electrophoresis analysis. But before he could do that, he needs to prepare 600 ml of 1x TAE from 95x TAE. How much 95x TAE and water should he add to create the 1x TAE solution? Afterwards, how much agarose does he need to weigh out, in grams, and add into 50 ml of 1x TAE?

Briefly explain the principle of SDS-polyacrylamide gel electrophoresis. What is the purpose of the SDS? Which...

Briefly explain the principle of SDS-polyacrylamide gel electrophoresis. What is the purpose of the SDS? Which proteins midrate slowest by SDS-PAGE?

How is PAGE (Polyacrylamide Gel Electrophoresis) different from other protein purification techniques?

What is the purpose of 1% agarose gel electrophoresis? Why is TAE buffer used during this...

What is the purpose of 1% agarose gel electrophoresis? Why is TAE buffer used during this procedure?

When given the basis of the genetic test, analyse an agarose gel electrophoresis result to genotype...

When given the basis of the genetic test, analyse an agarose gel electrophoresis result to genotype individuals or identify mutations.

Compare and contrast SDS-PAGE and Native-PAGE. Discuss the chemical and mechanistic similarities and differences between the...

Compare and contrast SDS-PAGE and Native-PAGE. Discuss the chemical and mechanistic similarities and differences between the two. Discuss why a scientist would prefer one method over the other.

3. What is the common label used to detect amplicons if you use agarose gel electrophoresis?...

3. What is the common label used to detect amplicons if you use agarose gel electrophoresis? 5. I ordered an 18-mer oligonucleotide primer from Fisher Scientific. What is an 18-mer? 8. You begin a PCR with 20 copies of the DNA template. After 5 cycles, approximately how many copies of amplicon do you have?

Question