Compare and contrast protein (SDS-PAGE) and DNA (agarose) gel electrophoresis.

Expert Solution

Difference between Protein (SDS-PAGE) gel electrophoresis and DNA (agarose) gel electrophoresis is as follows:

	Protein (SDS-PAGE) gel electrophoresis	DNA (agarose) gel electrophoresis
Name of gel used	Sodium dodecyl sulfate (SDS) polyacrylamide gel slabs is used.	Agarose gel is used.
Sepration	It is used for sepration of protein separation (Neutral PAGE can be used for DNA separation)	It is used for sepration of DNA and Nucleic acids.
Principle	It is based on Mass as well as charge.	It is based on the principle of charge and size of nucleic acids.
Resolution	When the PAGE gel (neutral) when used for DNA can give better resolution than Agarose gel.	It gives low resolution for DNA than SDS-PAGE gel.
Denaturation	In this electrophoresis proteins denatures prior to separation.	In this electrophoresis both denaturating and non denaturating technicques used.
Gel running	Always vertically.	Horizontally or vertically.
Uses	It is used for sepration of proteins based on their mass.	It is used for macromolecules using an electric field.

gladiator answered 1 year ago

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