Compare and contrast native gel electrophoresis and denaturing gel electrophoresis. Which method would be used to...

Compare and contrast native gel electrophoresis and denaturing gel electrophoresis. Which method would be used to determine the molecular weight of a protein, why?

Expert Solution

gladiator answered 2 years ago

Compare and contrast protein (SDS-PAGE) and DNA (agarose) gel electrophoresis.

Why would agorose gel will be inert in gel electrophoresis ?

Which of the following statement is FALSE? A. Gel electrophoresis is used to separate DNA according...

Which of the following statement is FALSE? A. Gel electrophoresis is used to separate DNA according to their size B. Chloroform is used for DNA precipitation C. PCR amplifies DNA to millions of copies D. Introns code for non-coding sequence whereas exons code for coding sequences Genotype frequency of the Alu insert in a population could be A. homozygotes (-/-) and homozygotes (+/+) B. heterozygous (+/-) and mongoloid population C. homozygotes (+/+) and asian population D. homozygotes (+/+) and mongoloid...

Compare and contrast membrane electrophoresis and capillary electrophoresis. What are the similarities and what are the...

Compare and contrast membrane electrophoresis and capillary electrophoresis. What are the similarities and what are the differences.

Describe capillary electrophoresis. Compare and contrast the method to HPLC. What is order of retention time?

For the gel electrophoresis system, what would be the consequence if the buffer added to the...

For the gel electrophoresis system, what would be the consequence if the buffer added to the outer chamber was higher than the lip of the outer plate?

Explain one other method (besides electrophoresis) that could be used to purify proteins and compare it...

Explain one other method (besides electrophoresis) that could be used to purify proteins and compare it to Nickel IMAC? P.S. Need to explain one method to separate and purify proteins but not electrophoresis, as we have done that in class.

Gel electrophoresis is a process used in laboratories to separate macro molecules. Mark the statements that...

Gel electrophoresis is a process used in laboratories to separate macro molecules. Mark the statements that are true as related to gel electrophoresis. An electrical charge is applied to the gel plate The pieces of DNA move due to electrical charge and also size A sample of the DNA is placed in a small well and a charge is applied The pieces of DNA or bands are stained to be visible Running a "gel" can be used to compare a...

How can DNA extraction, PCR, and gel electrophoresis be used in forensic science? Explain

how can gel electrophoresis be used to determine whether a ligation experiment was successful? Be specific...

how can gel electrophoresis be used to determine whether a ligation experiment was successful? Be specific with what you would expect to see in the various lanes of the gel if a ladder, comtrol ( digested plasmid + insert without ligase) and sample ( digested plasmid + insert with ligase) were run.

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