In: Chemistry
Q2. An enzyme has a Km of 2.8 * 10-5 M and a Vmax of 53 μM/s.
(i) Calculate Vo if [S] = 3.7 * 10-4 M and [I] = 4.8 *10-4 M for (a) a competitive inhibitor, (b) a noncompetitive inhibitor and (c) an uncompetitive inhibitor. (Assume Ki = 1.7 * 10-5 M.)
(ii) The degree of inhibition is given by i (%) = 100(1 - Vi/Vo). Calculate the percent inhibition in each of the three cases above.