Molecule ε260(M-1 cm-1) ε280(M-1cm-1) Protein 1 6219 11194 Protein 2 0 0 RNA A 102415 51207...

Molecule	ε260(M-1 cm-1)	ε280(M-1cm-1)
Protein 1	6219	11194
Protein 2	0	0
RNA A	102415	51207
RNA B	98623	49311

Protein 1 and RNA A are mixed in equimolar ratios and observed in a spectrophotometer with a 1 cm path length. The absorbance reading at 260 nm (A260) is 1.23. What would you anticipate A280 being for this sample?

3 mL of Protein 1 and 0.2 mL of RNA A were mixed to obtain the reading in Part A. What were the starting concentrations of Protein 1 and RNA A (in μM).

Solutions

Expert Solution

Starting concentration of protein 1 = 3770 micromolar.

Starting concentration of RNA A = 56500 micromolar.

queen_honey_blossom answered 1 year ago

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