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The molar absorptivity of a compound at 500 nm wavelength is 252 M-1cm-1. Suppose one prepares...

The molar absorptivity of a compound at 500 nm wavelength is 252 M-1cm-1. Suppose one prepares a solution by dissolving 0.00140 moles of a solute in enough water to make a 500.0 mL solution. What would be the absorbance in a 7 .00 mm pathlength cell? Type answer:  

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Expert Solution

The absorbance A of a solution of concentration C, molar absorptivity ε, and path length l is:

The molar concentration C is found using the following formula:

Given:

number of moles of solute = 0.00140 moles

Volume of solution = 500.0 mL = 0.5000 L

Therefore the molar concentration C is:

Therefore we have:

ε = 252 M-1cm-1

C = 0.00280 M

l = 7.00 mm = 0.700 cm

Therefore the absorbance of the solution is:

Therefore the absorbance of the solution is 0.494

queen_honey_blossom answered 2 years ago
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