The molar absorptivity of a compound at 500 nm wavelength is 252 M-1cm-1. Suppose one prepares...

The molar absorptivity of a compound at 500 nm wavelength is 252 M-1cm-1. Suppose one prepares a solution by dissolving 0.00140 moles of a solute in enough water to make a 500.0 mL solution. What would be the absorbance in a 7 .00 mm pathlength cell? Type answer:

Solutions

Expert Solution

The absorbance A of a solution of concentration C, molar absorptivity ε, and path length l is:

The molar concentration C is found using the following formula:

Given:

number of moles of solute = 0.00140 moles

Volume of solution = 500.0 mL = 0.5000 L

Therefore the molar concentration C is:

Therefore we have:

ε = 252 M-1cm-1

C = 0.00280 M

l = 7.00 mm = 0.700 cm

Therefore the absorbance of the solution is:

Therefore the absorbance of the solution is 0.494

queen_honey_blossom answered 1 year ago

Next > < Previous

Related Solutions

Oxy-myoglobin was measured at wavelength of 417 nm with molar absorptivity of 12,800 M^-1cm^-1. Met-myoglobin was...

Oxy-myoglobin was measured at wavelength of 417 nm with molar absorptivity of 12,800 M^-1cm^-1. Met-myoglobin was measured at wavelength of 409 nm with molar absorptivity of 17,900 M^-1cm^-1. In 10.0 g of meat (3.0 mL of total solution) the absorbance of oxy-myoglobin was 0.769 using a 1 cm cell and the met-myoglobin was 0.346. Find the moles of oxy-myoglobin and the met-myoglobin per gram of meat.

The molar absorption coefficients of tryptophan and tyrosine at 240 nm are 2.00 x 103 dm3mol-1cm-1 and 1.12 x 104 dm3mol-1cm-1 , respectively, and at 280 nm they are 5.40 x 103 dm3mol-1cm-1 and 1.50 x 103 dm3mol-1cm-1

The molar absorption coefficients of tryptophan and tyrosine at 240 nm are 2.00 x 103 dm3mol-1cm-1 and 1.12 x 104 dm3mol-1cm-1 , respectively, and at 280 nm they are 5.40 x 103 dm3mol-1cm-1 and 1.50 x 103 dm3mol-1cm-1 . The absorbance of a sample obtained by hydrolysis in a 1 cm cell was 0.660 at 240 nm and 0.221 at 280 nm. What are the concentration of the two amino acids? Without calculating, which amino acid is more abundant?...

Light with a wavelength of 500 nm (nano means one billionth or ) is incident upon...

Light with a wavelength of 500 nm (nano means one billionth or ) is incident upon a double slit with a separation of m. The screen is located 3 m away from the double slit. At what distance from the center of the screen will the ﬁrst bright fringe beyond the center-fringe appear?

A) Calculate the molar absorptivity constant at each concentration for all wavelengths B) Determine which wavelength...

A) Calculate the molar absorptivity constant at each concentration for all wavelengths B) Determine which wavelength you would use to run a calibration curve for the sample and defend the answer C) Explain why there are differences at each wavelength (if there are any) for the calculated molar absorptivity constant Path length for all exps = 2.5 cm Concentration Abs @ 315 nm Abs @ 320 nm Abs @ 325 nm Abs @ 330 nm Abs @ 335 nm 50...

What part of the spectra out provides information about the identity of the compound? Molar Absorptivity,...

What part of the spectra out provides information about the identity of the compound? Molar Absorptivity, Transmission , Absorbance, Wavelength What part of the spectra out provides information about the quantity of the compound? Molar Absorptivity, Transmission , Absorbance, Wavelength How is the measured quantity, transmittance, related to the calculated quantity, absorbance? Absorbance = 1/transmittance, Absorbance = e^(transmittance) , Absorbance = transmittance, Absorbance = -log(transmittance) How is absorbance related to concentration (the symbol α means proportional to)? Absorbance α concentration,...

3a. If the molar absorptivity constant for the red dye solution is 5.56×104 M-1 cm-1 ,...

3a. If the molar absorptivity constant for the red dye solution is 5.56×104 M-1 cm-1 , calculate the molarity of the red dye solution with absorption = 0.900. You may assume L = 1.22cm. Hint: A=εLC b. Convert the molarity in part a to w/v%. Show your work. Molar mass of FD&C red #3 = 879.86g/mol w/v% is defined as weight (in grams) of solute/100 mL of solution You should remember that w/v% always refers to the grams of materials...

A 0.50-mm-wide slit is illuminated by light of wavelength 500 nm.

A 0.50-mm-wide slit is illuminated by light of wavelength 500 nm. What is the width of the central maximum on a screen 2.0 m behind the slit in mm?

A 0.50-mm-wide slit is illuminated by light of wavelength 500 nm.

A 0.50-mm-wide slit is illuminated by light of wavelength 500 nm. What is the width of the central maximum on a screen 2.0 m behind the slit?

A 500 lines per mm diffraction grating is illuminated by light of wavelength 640 nm. 1....

A 500 lines per mm diffraction grating is illuminated by light of wavelength 640 nm. 1. What is the maximum diffraction order seen? Express your answer as an integer. 2. What is the angle of each diffraction order starting from zero diffraction order to the maximum visible diffraction order? Enter your answers in degrees in ascending order separated by commas.

Question: Light, with a wavelength of 500 nm in air, strikes directly on a double slit,...

Question: Light, with a wavelength of 500 nm in air, strikes directly on a double slit, spaced 440 micrometer apart. The resulting interference pattern is observed on a screen that is 2.0 meters away. Afterwards, the double slit apparatus is then submerged into oil, which has an index of refraction of 1.58. Assume that the diffraction effects are negligible. a)Calculate the distance between the first two adjacent bright fringes on the screen, when set up in air. b)Determine the frequency of...

Subjects