The molar absorption coefficients of tryptophan and tyrosine at 240 nm are 2.00 x 103 dm3mol-1cm-1 and 1.12 x 104 dm3mol-1cm-1 , respectively, and at 280 nm they are 5.40 x 103 dm3mol-1cm-1 and 1.50 x 103 dm3mol-1cm-1

The molar absorption coefficients of tryptophan and tyrosine at 240 nm are 2.00 x 103 dm3mol-1cm-1 and 1.12 x 104 dm3mol-1cm-1 , respectively, and at 280 nm they are 5.40 x 103 dm3mol-1cm-1 and 1.50 x 103 dm3mol-1cm-1 . The absorbance of a sample obtained by hydrolysis in a 1 cm cell was 0.660 at 240 nm and 0.221 at 280 nm. What are the concentration of the two amino acids?

Without calculating, which amino acid is more abundant?

Calculate the concentrations

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queen_honey_blossom answered 2 years ago

1.Tyrosine has an absorption peak near 280 nm with a molar extinction of 1,450. What would...

1.Tyrosine has an absorption peak near 280 nm with a molar extinction of 1,450. What would be the absorbance of tyrosine solution that is 1.5x10^-3 M in a 1 cm cell? in a 2 mm cell? in a 0.1 mm cell? 2. Using the molar extinction coefficient from problem 1, determine the E1%280 for tyrosine. The E1%280 is also an extinction coefficient but of a 1% solution measured at the peak near 280 nm. A 1% solution contains 1 gram...

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The molar absorption coefficients of tryptophan and tyrosine at 240 nm are 2.00 x 103 dm3mol-1cm-1 and 1.12 x 104 dm3mol-1cm-1 , respectively, and at 280 nm they are 5.40 x 103 dm3mol-1cm-1 and 1.50 x 103 dm3mol-1cm-1

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1.Tyrosine has an absorption peak near 280 nm with a molar extinction of 1,450. What would...