1) Design and draw a serial dilution to go from 4x10^8 cells/ml to 2x10^5 cells/ml. Indicate...

1) Design and draw a serial dilution to go from 4x10^8 cells/ml to 2x10^5 cells/ml. Indicate DF, TDF, and org/ml at each step.

Solutions

Expert Solution

Given concentration: 4*10⁸ cells/mL

The required concentration: 2*10⁵ cells/mL

The serial dilution can be done as shown below.

1^st dilution: 4*10⁸ * 1/2 = 2*10⁸ cells/mL, DF = 1: 3, TDF = 1: 3

2^nd dilution: 2*10⁸ * 1/2 = 1*10⁸ cells/mL, DF = 1: 3, TDF = 1: 3 * 1: 3, i.e. 1: 9

3^nd dilution: 1*10⁸ * 1/2 = 5*10⁷ cells/mL, DF = 1: 3, TDF = 1: 3 * 1: 3 * 1: 3, i.e. 1: 27

4^th dilution: 5*10⁷ * 1/2 = 2.5*10⁷ cells/mL, DF = 1: 3, TDF = 1: 3 * 1: 3 * 1: 3 * 1: 3, i.e. 1: 81

5^th dilution: 2.5*10⁷ * 1/5 = 5*10⁶ cells/mL, DF = 1: 6, TDF = 1: 3 * 1: 3 * 1: 3 * 1: 3 * 1: 6, i.e. 1: 486

6^th dilution: 5*10⁶ * 1/5 = 1*10⁶ cells/mL, DF = 1: 6, TDF = 1: 3 * 1: 3 * 1: 3 * 1: 3 * 1: 6 * 1: 6, i.e. 1: 2916

7^th dilution: 1*10⁶ * 1/5 = 2*10⁵ cells/mL, DF= 1: 6, TDF = 1: 3 * 1: 3 * 1: 3 * 1: 3 * 1: 6 * 1: 6 * 1: 6, i.e. 1: 17496

queen_honey_blossom answered 1 year ago

Next > < Previous

Related Solutions

Design a serial dilution to achieve a final dilution of 10^-4 such that there will be...

Design a serial dilution to achieve a final dilution of 10^-4 such that there will be enough solution remaining in each dilution to use for an assay that requires 100 mL of sample in triplicate. Remember that better accuracy is obtained with relatively large dilutions if the total dilution is made from a series of smaller dilutions rather than one large dilution.

1. Sketch a connected 2-dimensional complex with three 2-cells, 8 1- cells, and 5 0-cells. Draw...

1. Sketch a connected 2-dimensional complex with three 2-cells, 8 1- cells, and 5 0-cells. Draw three examples of a connected 1-dimensional complex with 7 0-cells and 6 1-cells. Sketch a connected 1-dimensional complex with 7 0-cells and 7 1-cells. Sketch a connected 1-dimensional complex with 7 0-cells and 8 1-cells. Is it possible to construct a connected 1-dimensional complex with 8 0-cells and 6 1-cells? Explain.

Acetic Acid Serial Dilution For part of the experiment, you will need to prepare 100 mL...

Acetic Acid Serial Dilution For part of the experiment, you will need to prepare 100 mL of 0.025 M CH3CO2H (acetic acid), starting from commercial “glacial” acetic acid, which has a concentration of 17.4 M. Devise a method using a two-step serial dilution to make 100 mL of 0.025 M acetic acid solution. The glassware available is a 100 mL volumetric flask, 50.00 mL volumetric flask, 1.00 mL volumetric pipet, and a 10 mL graduated pipet. For the first step,...

A student was given 6 plates taken from the incubator from a serial dilution done two...

A student was given 6 plates taken from the incubator from a serial dilution done two days earlier and told to get a standard plate count. The student, not sure what to do, attempted to count the colonies on all the plates, then add the counts from all plates and get an average. Was this the correct procedure? Yes or No? Explain!

Construct a table to show how to make the standard solutions from serial dilution, starting with...

Construct a table to show how to make the standard solutions from serial dilution, starting with a stock solution of 1000 ppm in 100 mL volumetric flask. Use 1000 ppm standard soltuions of Co, Cr, Pb, Cd, and Zn to prepare the following five standard solutions with 10% HNO3 in 100 mL volumetric flask: Std 1: 100 ppm of Co, Cr, Pb, Cd, Zn Std 2: 50 ppm of Co, Cr, Pb, Cd, Zn Std 3: 10 ppm of Co,...

Complete a 1:5 dilution series with each tube containing a final bolune of 10mL. Indicate the...

Complete a 1:5 dilution series with each tube containing a final bolune of 10mL. Indicate the dilution factor of each tube relative to the stock and the volume of water and solution in each tube. The first tube represebts the concentrated stock solution, make dilutions in the reaminubg for tubes. Please show work

How would set up a serial dilution to cover a concentration range from approximately 21 µM...

How would set up a serial dilution to cover a concentration range from approximately 21 µM to 120 µM of DHF in anassay (in the well) having a total volume of 200 µL of which 30 µL is DHF solution? You are provided a 800 µM DHFstock solution. You need to make a set of DHF dilutions in Eppendorf tubes first. From these you will take 30 µL eachand dilute into total volume of 200 µL in the wells can...

You counted 620 colonies on a plate with a dilution of 10-2. How many cells/ml were...

You counted 620 colonies on a plate with a dilution of 10-2. How many cells/ml were in the original sample?

In 2-3 sentences, describe howto do a serial dilution of a 1x 10-3Msolution to obtain 1...

In 2-3 sentences, describe howto do a serial dilution of a 1x 10-3Msolution to obtain 1 L of a 1x 10-9M solution. (Include necessary volumes and equipment.)

2. A serial dilution of patient serum is done: Tube #1 Tube #2 Tube #3 Tube...

2. A serial dilution of patient serum is done: Tube #1 Tube #2 Tube #3 Tube #4 Tube #5 Tube #6 1:2 1:4 1:8 1:16 1:32 1:64 One milliliter of RBCs is added to each tube and the following is noted: Tube #1 No agglutination Tube #2 No agglutination Tube #3 Agglutination Tube #4 Agglutination Tube #5 Agglutination Tube #6 Agglutination a. What has happened in Tubes #1 and 2? b. What is the antibody titer? 3. You suspect that...

Subjects