In: Chemistry
Create a calibration curve and line of best fit using this information and Beer's Law
Volume of Stock Solution (ml) |
Concentration |
Absorbence |
1.00 |
2.066 |
0.121 |
2.00 |
1.969 |
0.254 |
5.00 |
1.981 |
0.631 |
7.00 |
2.044 |
0.856 |
See what you need to do is simply multiply concentration with volume of stock solution in Litres.
That is what is shown below in the third column. 1ml = 0.001 L
Then you plot graph of this quatity which is infact the number of moles of solute in solution vs the absorbace in the Y axis, you get a straight line.
I have taken moles as you havent specified the total volume in which all these solutions are made, so if you plan to use this calibration curve, make sure you use the same conditions as you used to prepare the standard solutions
Also to make thinhgs better, you can always set intercept as zero for zero conc, as you know this is always true as long as solvent does not adsorb.