Question

What use's are there for Beer's law? What, if any, limitations are there on using Beer's law? Are there common, everyday examples where Beer's law can be observed or is used?

Answer 1

According to Beer's-Lamberts law, Absorption of the sample depends on the concentration of the analyte and the path length of the tube. This can be mathematically represented as follow's

A =ε * b * c

where, ε is the molar extinction coefficient.

The general use as follows,

The above procedure is the example for the laboratory use of everyday.

Limitations:

-----> Devitaion of abosorption when we use the concentration of the sample exceeds 0.01M.

------> scattering of light due to the presence of particulates in the analyte.

------> Analyte may undergo fluoresecence or phosphorescence.

------> Change in the refractive index due to the high concentration of the analyte.

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