In an indirect colorimetric assay such as the biuret assay, the color-forming reagent must be present...

In an indirect colorimetric assay such as the biuret assay, the color-forming reagent must be present in excess. Why ?

Expert Solution

Thus, the color-forming reagent must be present in-excess.

queen_honey_blossom answered 2 years ago

what is the chemical principle of the Biuret assay (i.e. what reacts with what)? Are there...

what is the chemical principle of the Biuret assay (i.e. what reacts with what)? Are there any known interferences or biases in this assay? Draw a scheme of the biuret reaction

A colorimetric LDH assay provides a method to measure the enzyme activity of LDH. NADH (MW...

A colorimetric LDH assay provides a method to measure the enzyme activity of LDH. NADH (MW 633.4 g/mol) absorbs light at 340 nm while NAD+ does not show any absorption at that wavelength. The Beer-Lambert Law relates the absorbance of a solute to its concentration: A = ε*c*L A is absorbance, ε is the molar extinction coefficient, c is concentration, and L is the path length of the solution in the spectrophotometer (usually in cm). In the lab you have...

Use the Beer-Lambert law to explain how a colorimetric assay works in the spectrophotometer. In your...

Use the Beer-Lambert law to explain how a colorimetric assay works in the spectrophotometer. In your answer, define the following terms; chromophore, extinction coefficient, absorbance, transmission and reagent blank. Most of the spectrophotometers in the teaching lab emit light of wavelengths between 400-700nm. However some spectrophotometers also emit light between 200-400nm. Why is this extended range useful? Provide a specific example. How can you use a spectrophotometer to perform a qualitative analysis of a substance?

A technologist is performing a colorimetric assay using 96?well ELISA microtiter plates. The ELISA plate reader...

A technologist is performing a colorimetric assay using 96?well ELISA microtiter plates. The ELISA plate reader measures the absorbance of the solution in each well (cuvet) by recording the light passing through the solution from the top to the bottom of each well. The assay’s color, when tested in a standard spectrophotometer, is linear to an absorbance of 4.0 however the ELISA reader has a range of linearity which only extends to an absorbance of 1.2. When a microtiter plate is placed...

The development of leaders within the company present this in an assay format

In an HIV Test using indirect ELISA, (a) explain the principle of the assay (b) what...

In an HIV Test using indirect ELISA, (a) explain the principle of the assay (b) what is not included in the negative control for indirect ELISA when testing for HIV?

Why would CuSO4 have a blue color after the iron reagent is added? Yes, the answer...

Why would CuSO4 have a blue color after the iron reagent is added? Yes, the answer is because it is absorbing the other colors (the spectrophotometer), but WHAT is absorbing the other colors? (Done in an experiment about spectrophotometry)

A visible light spectrophotometric determination of penicillin makes use of color-forming reaction of the penicillin. If...

A visible light spectrophotometric determination of penicillin makes use of color-forming reaction of the penicillin. If this compound absorbs at 475 nm with an ε = 1560 M-1cm-1, what would be calculated as the limit of detection with an instrument, using a 1.00 cm cuvette, with its smallest absorbance measurement at 0.005?

Potassium ferricyanide illustrates the presence of which ion by forming the characteristic blue color called Prussion...

Potassium ferricyanide illustrates the presence of which ion by forming the characteristic blue color called Prussion blue? What is significant about the presence of this ion?

How would the signal intensity (amount of color detected) in an indirect IHC experiment compare to...

How would the signal intensity (amount of color detected) in an indirect IHC experiment compare to the signal intensity of direct IHC. Explain your answer.

Question