How would the signal intensity (amount of color detected) in an indirect IHC experiment compare to...

How would the signal intensity (amount of color detected) in an indirect IHC experiment compare to the signal intensity of direct IHC. Explain your answer.

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- In direct detection methods, the primary antibody is directly conjugated to a label. During indirect detection, the primary antibody is bound by a labeled secondary antibody that has been raised against the host species of the primary antibody.
- Indirect method use to increase signal intensity.
- Direct detection is suitable for detecting highly expressed antigens. For direct detection, the primary antibody can be conjugated to an enzyme.
- Benifits of direct detection is increased flexibility in the design of multicolor experiments.
- Indirect detection is more suitable for studies of poorly expressed antigens, which benefit from the signal amplification provided by the secondary reagent .

gladiator answered 2 years ago

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How would the signal intensity (amount of color detected) in an indirect IHC experiment compare to...

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