In an HIV Test using indirect ELISA, (a) explain the principle of the assay (b) what...

In an HIV Test using indirect ELISA,
(a) explain the principle of the assay
(b) what is not included in the negative control for indirect ELISA when testing for HIV?

Expert Solution

gladiator answered 5 months ago

. Which of the following best matches the description: a. ELISA b. indirect fluorescent antibody test...

. Which of the following best matches the description: a. ELISA b. indirect fluorescent antibody test c. Ouchterlony procedure d. Western blot e. fluorescent activated cell sorter 1. nylon filter 2. flow cytometry 3. fluorescent microscope 4. chromogen 5. agarose gel

Describe an ELISA test to detect the presence of HIV antibodies in a patient.

From the technical instruction of ELISA method, please suggest how the ELISA HIV test kit was...

From the technical instruction of ELISA method, please suggest how the ELISA HIV test kit was prepared?

Describe three ways that an immunofluorescence assay is similar to an ELISA that would test for...

Describe three ways that an immunofluorescence assay is similar to an ELISA that would test for specific antibodies.

Describe three ways that an immunofluorescence assay is different to an ELISA that would test for...

Describe three ways that an immunofluorescence assay is different to an ELISA that would test for specific antibodies.

what is a) What is ELISA? Briefly explain the sandwich ELISA technique. b) What is a...

what is a) What is ELISA? Briefly explain the sandwich ELISA technique. b) What is a Rhogram?

A technologist is performing a colorimetric assay using 96?well ELISA microtiter plates. The ELISA plate reader...

A technologist is performing a colorimetric assay using 96?well ELISA microtiter plates. The ELISA plate reader measures the absorbance of the solution in each well (cuvet) by recording the light passing through the solution from the top to the bottom of each well. The assay’s color, when tested in a standard spectrophotometer, is linear to an absorbance of 4.0 however the ELISA reader has a range of linearity which only extends to an absorbance of 1.2. When a microtiter plate is placed...

Describe 2 ways that an immunofluorescence assay is different from an ELISA that would test for...

Describe 2 ways that an immunofluorescence assay is different from an ELISA that would test for specific antibodies.

Explain the difference between nonspecific binding and specific binding using an indirect ELISA as an example....

Explain the difference between nonspecific binding and specific binding using an indirect ELISA as an example. For this assay, assume that you are determining the titer of antibody against BSA. You bind BSA to wells of a microtiter plate, add a dilution series of the antibody in question across the wells of the plate, then detect that antibody with an enzyme-conjugated antibody/chromogenic substrate pair.

1)ELISA is widely used as the initial screening test for diseases such as HIV, lupus, and...

1)ELISA is widely used as the initial screening test for diseases such as HIV, lupus, and Lyme disease. How would western blot be used in the diagnostic workflow? 2) Why are Precision Plus Protein Kaleidoscope standards run alongside the protein samples in the gel? 3) In a complex organism, is it possible for a single gene to encode for multiple proteins? If yes, explain.

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