A good reason to increase flow rate in chromatography is Choose the right answer (a) to...

A good reason to increase flow rate in chromatography is

Choose the right answer

(a) to minimize eddy diffusion.

(b) to minimize column bleed.

(c) to minimize brand broadening due to longitudinal diffusion.

(d) to minimize brand broadening due to mobile phase mass transport.

Expert Solution

c) to minimize band broadening due to longitudinal diffusion.

Eddy diffusion is caused by turbulence in solute flow and is not affected by flow rate. Column bleed is primarily caused due to contamination and flow rate doesn't affect it much.

Band broadening due to mobile phase mass transport exhibits H as directly proportional to the flow rate. Hence if flow rate increases,band broadening due to mobile phase mass transport increases.

Band broadening due to longitudinal diffusion is inversely proportional to the flow rate. Hence as flow rate increases, band broadening due to longitudinal diffusion decreases.

queen_honey_blossom answered 2 years ago

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