Compare and contrast Column (solid-liquid) chromatography, Paper
Chromatography, and thin layer chromatography in respect to the
size of the sample that can be separated, the ease of use, and the
versatility of the method.
Compare the advantages and limitations of column chromatography
with those of thin-layer and gas chromatography. Discuss all 3
chromatography methods. When you would use them and in which case
is each useful and not useful?
Thin Layer Chromatography utilizes a glass or metal plate coated
with a thin layer of absorbent material such as silica gel. The
sample is prepared similiarto paper chromatography and a solvent is
used to elute the sample.
1. Which is the stationary phase? Plate Silica
Gel Solvent
2. Which is the mobile phase? Plate Silica
Gel Solvent
3. Which component is inert? Plate Silica
Gel Solvent
Explain thin layer chromatography and its utility in determining
the components of a mixture. Be sure to briefly explain how TLC
works as well as what and how the visualization techniques
work.