500ml of an HTS/TS- buffer (pKa = 5.25) at pH 4 was prepared. Unfortunately, the student...

500ml of an HTS/TS- buffer (pKa = 5.25) at pH 4 was prepared. Unfortunately, the student who had prepared the buffer forgot how many moles of HTS and TS- he had added. Although HTS does not fluoresce, TS- does fluoresce. (If = 1000CTS). The intensity of the fluorescence signal from the buffer is 10. How many moles of HTS and TS- were used to prepare 500ml of the pH 4 buffer?

Expert Solution

queen_honey_blossom answered 2 years ago

A pH 3.30 buffer is prepared from iodoacetic acid ICH2COOH (pKa = 3.175), and sodium iodoacetate,...

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