plot 1/Vo Vs 1/[S] (Lineweaver-Burk plot) and calculate the values of Km and Vmax from the...

plot 1/Vo Vs 1/[S] (Lineweaver-Burk plot) and calculate the values of Km and Vmax from the intercepts .Give units

Expert Solution

queen_honey_blossom answered 2 years ago

I need to calculate the turnover rate of an enzyme using a lineweaver-burk plot. Is there...

I need to calculate the turnover rate of an enzyme using a lineweaver-burk plot. Is there a specific equation that should be used to calculate Kcat? I have been unable to find a straightforward answer on how to calculate Kcat!

When Vo = 0.5× Vmax, [S] = 1× Km. Which of the following is true when...

When Vo = 0.5× Vmax, [S] = 1× Km. Which of the following is true when [S] = 4× Km? V0 = 40% of Vmax none of these V0 = 8% of Vmax Vo = 80% of Vmax Vo = 4% of Vmax

Do the required calculations and graph the results as a Lineweaver-Burk plot.

Carbonic anhydrase catalyzes the reverse reaction (of course!): HCO3 - + H+  CO2 + H2O Initial velocities were measured at the following concentrations of HCO3 - (The enzyme concentration was 0.0025 mg/ml; the enzyme Mr = 29,100 Da.): [HCO3 -] mM 1.3 2.6 6.5 13.0 26.0 vo (µM/sec) 2.5 4.00 6.30 7.60 9.00 vo (µM/sec) with acetazolamide 1.17 2.10 4.00 5.70 7.20 a. Do the required calculations and graph the results as a Lineweaver-Burk plot. Use the X- and...

5. a. In a Lineweaver-Burk plot, the y-intercept of the line of best fit is equal...

5. a. In a Lineweaver-Burk plot, the y-intercept of the line of best fit is equal to _____ and the x-intercept of the line of best fit is equal to _____: 1) 1/Vmax ; –1/Km 4) –1/Km ; Vmax 2) 1/Km ; 1/Vmax 5) Vmax ; Km 3) 1/Vmax ; –Km b. All of the following statements are true about the relationships between [S], Km, Vo, and Vmax EXCEPT: 1) As [S] increases, Vo gets closer to Vmax 2) Vmax occurs when...

BIOCHEMISTRY: Vmax and Km values For the following problem

Condsider five conditions of some enzyme(s) with the following Lineweaver-Burk equations. A) y = 1x +...

Condsider five conditions of some enzyme(s) with the following Lineweaver-Burk equations. A) y = 1x + 0.25 B) y = 0.25x + 0.25 C) y = 1x + 1 D) y= 1x + 4 E) y= 0.25x + 1 i) Which set of conditions demonstrates the enzyme that is least able to form the ES complex easily? v) Which pair of sets of conditions would represent an enzyme with and without a competitive inhibitor? vi) Which pair of sets of...

How does a competitive inhibitor change the Km and Vmax values for an enzyme such as...

How does a competitive inhibitor change the Km and Vmax values for an enzyme such as Penicillin?

An enzyme catalyzed reaction has a Km of 3.76 mM and a Vmax of 6.72 nM/s....

An enzyme catalyzed reaction has a Km of 3.76 mM and a Vmax of 6.72 nM/s. What is the reaction velocity in nM/s, when the substrate concentrations are: A. 0.500 mM B. 15.6 mM C. 252 mM D. Now assume you have added a competitive inhibitor that has a concentration of 15.6 µM with a KI of 7.30 µM to the enzyme in question 9. Calculate the velocity at the same substrate concentrations as above: E. 0.500 mM F. 15.6...

How will you describe and contrast on enzyme activity based on Km and Vmax from Michaelis...

How will you describe and contrast on enzyme activity based on Km and Vmax from Michaelis menten equation? (low or high Km/Vmax) what does it mean when Km is High or low what does it mean when Vmax is High or Low

Using S? values from Appendix C in the textbook, calculate ?S? values for the following reactions....

Using S? values from Appendix C in the textbook, calculate ?S? values for the following reactions. 5 significant figures please!!! C2H4(g)+H2(g)?C2H6(g) N2O4(g)?2NO2(g) Be(OH)2(s)?BeO(s)+H2O(g) 2CH3OH(g)+3O2(g)?2CO2(g)+4H2O(g)

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