Question

. Which of the following best matches the description:

a. ELISA

b. indirect fluorescent antibody test

c. Ouchterlony procedure

d. Western blot

e. fluorescent activated cell sorter

1. nylon filter

2. flow cytometry

3.  fluorescent microscope

4. chromogen

5. agarose gel

Answer 1

1. ELISA Test - Chromogen

2.Indirect Fluorescent Antibody Test - Fluorescent microscope

3.Ouchterlony procedure - Agarose gel

4. Western blot - Nylon Filter

5.Fluorescent Activated Cell Sorter - Flow Cytometry

1. ELISA Test - Chromogen

In the direct ELISA, antigens are immobilized in the well of a microtiter plate. An antibody that is specific for a particular antigen and is conjugated to an enzyme is added to each well. If the antigen is present, then the antibody will bind. After washing to remove any unbound antibodies, a colorless substrate (chromogen) is added. The presence of the enzyme converts the substrate into a colored end product.

2.Indirect Fluorescent Antibody Test - Fluorescent microscope

Indirect fluorescent antibody (IFA) test can be used to detect specific antibodies against various etiological agents present in patient serum and cerebral spinal fluid (CSF) samples. The smear is visualised through the Fluorescent microscope.

3.Ouchterlony procedure - Agarose gel

Ouchterlony procedure is an Immuno-diffusion technique used for the detection or measurement of antibodies and antigens by their precipitation which involves diffusion through a substance such as agar or gel agarose. Simply, it denotes precipitation in gel.

4. Western blot - Nylon Filter

In Western Blot, selecting the proper membrane is critical to the success of protein transfer procedure. Nylon membranes are used in western blot test. They have high mechanical strength, which offers benefits in western blot experiments requiring stripping and reprobing procedures. Nylon membranes consistently provide a higher efficiency of protein transfer SDS gels than nitrocellulose membranes.

5.Fluorescent Activated Cell Sorter - Flow Cytometry

Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell.