Question

Medical testing: enzyme linked immunosorbent assay (ELISA) test for presence of antigen or antibody

EXPERIMENTAL PROTOCOL

In this laboratory, you will test seven different patients using a direct ELISA. We have access to serum samples from the main characters of the TV show The Big Bang Theory. You will test each of them for the presence of ZIKA virus and HIV, as well as perform a pregnancy test, using known antigens/antibodies for those diseases/test. Students will work in pairs. Serum for each character of the Big Bang Theory has been incubated overnight at 4°C in the wells of an ELISA plate.

Testing	Control	Sheldon	Penny	Leonard	Raj	Bernadetts	Howard	Amy
Zika		+		+	+		+
hCG (pregnancy)			+					+
HIV			+		+

1) Interpretations: Summarize the interpretation of the findings about the results and the experiment

2) Future Scientific Directions: suggest future experiments using as base the results obtained in the lab

3) Create a question to the lab experiement approach.

5)  Approach: Create a summary of the overall lab experiment. Hypothesis.

Answer 1

1) In the above experiment pateint saples were tested to for the presence of Zika virus , HIV and hcG horome for pregnancy. Among seven pateint sample, 4 pateint get ZIKA virus, two of them showed HIV and two of the pateint were positive for human .

2) Once the pateint sample showed presence of viruses like ZIKA or HIV in future the samples can be analysed for how the other serum parameters get affected by the presence of these viruses.

3) can we detect the presene of viruses such as HIV or ZIKA by enzyme linked immunosorbent assay (ELISA)?

4) enzyme linked immunosorbent assay (ELISA) test is perform to detect the presence of viruses ´like ZIKA or HIV. In this method antigen for ZIKA or HIV are coated on the walls of ELISA. When the pateint blood contains the antibody against ZIKA or HIV they will bind to the antigen and labeled HRP and substrate will then used to detect the presene of specific viruse. Similarly to perform the ELISA for hCG, wells are coated with antigen against hCG and if the pateint blood has the antibody for hCG it will be detected by the antigen-antibody reaction.

HYPOTHESIS: Enzyme linked immunosorbent assay (ELISA) test can be used to detect the presence of specific antigen or antibody in pateint sample.