Question

The average risk of transmission of the human immunodeficiency virus (HIV) to a health care worker after percutaneous exposure to HIV-infected blood has been estimated as 0.3 percent. However, the factors that influence this risk have not been determined, and the efficacy of postexposure prophylaxis with antiretroviral drugs have not been clinically evaluated. If postexposure prophylaxis is effective, it would offer an entirely new strategy for preventing HIV transmission in nonoccupational settings as a supplement to the preferred strategy of preventing exposure. Study of occupational exposure to HIV presents an important opportunity to evaluate postexposure prophylaxis, because the source, time, and many details of the exposure are known. A nationwide, prospective, placebo-controlled trial of prophylaxis with zidovudine after percutaneous exposure to HIV among health care workers was discontinued when only 84 health care workers enrolled after one year since many thousands would be needed to assess the reduction of a 0.3 percent risk of transmission. Nevertheless, occupational exposure to HIV and infection continue to occur, and there is a compelling public health need for data on the efficacy of postexposure prophylaxis. Why HIV consider a very dangerous infection? discuss in detail. Discuss the course of infection ( infection, incubation period, Aids syndrome). What is the new treatment plans for HIV?

Answer 1

Virtually created panic among health care workers about pandemic acquired immune deficiency syndrome prompted us to review the scientific literature to investigate the risk of human immunodeficiency virus (HIV) transmission in the daily works of health care workers, especially surgeons and anesthesiologists. In this review, we report worldwide valuations of the number of HIV infections that may occur from unsafe daily work in health care. We also present how to minimize the risk of infection by taking precautions and how to utilize postexposure prophylaxis in accordance with the latest reports of the Centers for Disease Control and Prevention. HIV-infected patients will be aging, and most of them will become the candidates for procedures such as major vascular reconstruction and artery bypass grafting, where the risks of blood contact and staff injury are high. For these reasons, all health care workers need to know how to prevent, and fight following the accidental exposure to HIV.

Human immunodeficiency virus (HIV) or immunodeficiency virus that leads to acquired immunodeficiency syndrome, called AIDS, is transferred primarily via sex contacts and exposition to infected blood or its ingredients. It is estimated that currently 32.2–38.8 million human beings are HIV-positive globally.

1) Because of risky behaviors, heterosexuality, development of civilization, and declining boundaries between people, a chance of HIV contagion is a real threat in everyday life. This is particularly true of health care employees including surgeons and anesthetists. Despite a decline in the proportion of newly reported infections of HIV from 3.4 to 2.3 million from 2001 until 2012 per year, which is 33%, due to medical advances and longer survival of people, approximately one-fourth of the HIV-positive patients will need surgical and/or anesthetic treatment in the future.

2) It should be expected that in the next few years the percentage of HIV patients requiring surgical and anesthetic treatment will be increased further.

HIV history and epidemiology

The newly discovered complex disease that was named acquired immunodeficiency syndrome was described in 1981 in a homosexual patient living in the USA, who died of Pneumocystis carinii pneumonia. In 1983, the pathogen that leads to AIDS, the HIV, was identified and named. Luc Montagnier with his working group (1983) was the first who isolated HIV, at the Pasteur Institute in Garches, near Paris. In 2008, Luc Montagnier, together with Françoise Barré-Sinoussi, was awarded The Nobel Prize in Physiology or Medicine for this discovery. Regarding HIV and AIDS, we discuss about a pandemic, which is one of the numerous epidemics of HIV infections in different environments, in different continents at the same time, which differ in the number of patients and the risk of infection on the basis of latitude, type of population, and behaviors.

HIV is a Lentivirus that belongs to a group of retroviruses. There are two variants of this virus, HIV1 and HIV2, which occur primarily in West Africa. The reverse transcriptase enzyme, in which the retroviruses are equipped, enables them to transcribe their own RNA into DNA and then merge them to host cell genome. The HIV attacks the cells containing antigens CD4 (T-helper lymphocytes and macrophages) on their surface and then destroys them, which leads to immunodeficiency, increased incidence of opportunistic infections, and malignancies.

HIV risk among health care workers

HIV was isolated from different body fluids, for example, urine, semen, cerebrospinal fluid, blood, tears, amniotic fluid, saliva, breast milk, and vaginal secretions. However, epidemiologists report that HIV infection occurs mainly through sexual contact, contaminated blood, breast milk, semen, and vaginal secretions.It has been proved that blood is the major and the strongest source of infection of not only HIV but also other pathogens and is the major route of transmission in health care employees.On the other hand, infection through semen and vaginal secretions for health care workers seems to be irrelevant, because contact with these fluids is minimal, and even during contact, protective gloves are worn during patient examinations, which are sufficient to prevent infection. Also, infection through cerebrospinal fluid, synovial fluid, and peritoneal or pleural fluid seems to be very unlikely. While searching the global literature, we found one case of HIV infection during puncture and evacuation of bloody fluid from the pleural cavity.Unfortunately, the risks associated with HIV transmission and other pathogens through the aforementioned body fluids is not certain due to insufficient epidemiological data. The risk of HIV transmission through body fluids such as urine, sputum, feces, vomits, nasal secretions, sweat, and tears is low or even does not exist if they do not contain visible blood. However, these fluids are also a source of transmission for other pathogens that are dangerous to human health. Human milk can also be a potential risk factor for the infection of blood-borne viruses, including HIV. However, it is not a risk factor for health care workers (except nursing neonate), especially surgeons or anesthesiologists, because of very low or even no exposure to this type of body fluid. Also, saliva of patients infected with the HIV poses no threat to the daily works of surgeons and anesthetists. This was demonstrated by epidemiological data gathered from studies among families living with a HIV-positive member, where HIV was not transmitted to other family members, despite the contamination of open wounds with saliva from the infected family member. However, that saliva is a potential source of infection of hepatitis B virus (HBV).

Henderson conducted an ongoing prospective study for 6 years in order to estimate the risk of HIV transmission among health care workers and HIV infection risk associated with the exposure to various risk factors. The median follow-up of health care workers subjected to parenteral exposure to a potential source of HIV infection in the course of their activities was 30.2 months (from 6 to 69 months). In the studied group of 1,344 health care employees, 179 reported percutaneous and 346 mucous membrane exposition to potentially infectious body fluids from a person infected with HIV. After sending an additional questionnaire to 559 employees, it is found that 2,720 reported cutaneous exposure to infected blood with HIV. Also, over 10,000 health care workers reported skin exposure in health care workers to blood from all patients over 12 months. Transmission of HIV was observed in one employee after parenteral exposure to blood from a HIV-positive patient. There was no infection after exposure to HIV-infected blood at the mucosa or skin. Summing up the test results, Henderson et al16 estimated that the risk of transmission of HIV infection related to percutaneous exposure to infected blood is 0.3% for each exposure. The Center for Disease Control and Prevention, Atlanta, estimates one HIV seroconversion for every 200 contaminated needlesticks. From the literature, we can also find that the probability of infection of HIV by needle injury is in the range from 0.3% to 0.03%, which increases with the depth of injuries, volume of inoculated blood, and hollow needle injuries. The probability of HBV infection after injury with contaminated needle with blood from HBeAg-positive patient is much higher than in the case of HIV and is 6%–30% versus 0.3%. At least one needle stick injury occurs in 20% of anesthesiologists during a 3-month work period, in their 30 years of work, and the overall risk of needle injury is 4.5%. During central vein cannulation, 90% of the anesthesiologists use gloves, whereas during a peripheral venous cannulation, only 8% use gloves, which should be 100% in each case. On the other hand, the risk of infection associated with the exposure of mucous membranes to the HIV-infected blood is 0.09%. Hussain documented that the risk of accidental injury to staff during surgery is 5.6% and that the risk is higher in long operations and at the time of laparotomy wound closure. Lowenfels reported a lower accidental injury rate in operating room staff than Hussain et al, but their figure was based on retrospective estimates.

Marnejon et provide more information regarding the exposure of residents to accidental injury during postgraduate education. In 2000–2014, Marnejon et al recorded 129 needlestick and sharps injuries among the studied group of 924 residents. Approximately 62.7% of needlestick and sharps injury occurred in the first 6 months during the first-year postgraduate education. The most vulnerable groups of residents were dental, obstetrics and gynecology, and surgery residents, where exposure to needlestick or sharps injury was 30.6%, 28.9%, and 18.5%, respectively. The left index finger (19.4%) and the left middle finger (16.9%) were the frequently damaged. Approximately 43.4% of the injuries occurred while using suture needles, 11.6% while using of scalpels, and 10.1% while using blood syringes. Fortunately, there was no seroconversion in any of the cases, although 16 patients were infected with hepatitis C virus (HCV) and one with HBV.

Most of the people with AIDS exhibited high-risk behaviors which led to HIV infection. This also applies to health care workers whose risky behaviors are the cause of 95% of HIV infections, and the cause for the remaining 5% of transmission of HIV infection has not been determined. In workers of professions other than medical, only 3% of the employees cannot clearly determine the cause of HIV infection.The daily routine of health care workers, including surgeons and anesthetists, leads to the fact that they do not treat every patient as a potential HIV-positive. Only when the patient is from the risk group (male homosexuals and bisexuals, intravenous drug abusers, hemophiliacs, and the child of parents who are HIV-positive), they start to take precautions. Kelen clearly show that relying on clinical suspicions is insufficient to recognize patients from these groups and that the surgeons who are relying on clinical suspicion will be underestimating the numbers of their patients at risk.

Many surgeons and anesthesiologists think that HIV testing should be performed before surgery or any other invasive procedure. In addition, a negative HIV test in a patient at risk group could reduce vigilance and care of health care workers by the conviction that there is no risk in such patients, who still exhibit high-risk behavior and who in the recent past could have become HIV-positive or is in the serological window. The long incubation time between HIV infection and clinically recognizable sequelae such as AIDS will result in a growing population of asymptomatic HIV carriers. Simultaneously, the cohort of infected patients will be aging and more will become candidates for procedures such as major vascular reconstruction or artery bypass grafting, where the risks of blood contact and staff injury are high. Therefore, it is necessary to realize that every patient can be HIV-positive or can be infected with other blood-borne pathogens; hence, it is very important to apply safety rules in each day for all patients during each medical procedure.

Preventing infections

The most important precaution is washing hands before and after examining a patient, which is the most often ignored precaution among the medical staff. Also, it is necessary to immediately wash the other parts of the body, if they have been soiled with any body fluid from the patient. Using disposable gloves during every examination of the patient reduces the risk of infection of all pathogens. It also reduces the transmission of any pathogen to the patient from hands of the doctor or the nurse while performing a medical procedure. Global data show that the probability of HIV transmission from a health care worker to a patient is extremely rare and varies depending on the source from 2.4–24 per million medical procedures. Wearing double gloves during surgery reduces contamination of the skin by blood, tissue, and other fluids from the patient to the entire surgical team, especially the surgeon. However, using double gloves is not the general rule. Some surgeons explain that they do not use double gloves because of the discomfort or loss of sensitivity during the surgical procedure. Miles reported that 26% of surgeons during an operation removed the second pair of gloves because of discomfort or loss of sensitivity in their hands. While taking blood samples, while changing drips, or in any other situation where there is a possibility of contact with blood or other body fluids, medical personnel should wear protective gloves.

Needlesticks occur particularly when a suture is being placed through tissue directly supported by the opposite hand. This maneuver can be avoided or a thimble or other protective shield can be placed over the distal left index finger (in right-handed surgeons), which is the most often penetrated site. A simple precaution is tying sutures in tissues after cutting needle, which reduces the risk of needlestick injuries with the small costs of more suture usage. Also, surgeon can use staples for bowel anastomosis and skin closure. However, this increases the cost of surgical procedure.

Undoubtedly, wearing protective glasses reduces chance of contamination of the conjunctiva with blood during surgical procedure. Unfortunately, conventional spectacles provide partial but acceptable protection, although they are open on the sides. Brearley and Buist  found that in 25% of operations in general surgery, there is at least one splash of blood on the protective glasses from the patient. According to their study, of 257 operations, in only three cases, the surgeons were aware of the contamination. On the other hand, Porteous points out that the type of the operations have an impact on more or less risk of contamination of the conjunctiva with blood or other tissue from the patient. Hence, joint arthroplasty seems to be a high-risk procedure, with the risk of contamination of the conjunctiva with blood compared with dynamic hip screw insertions (84% versus 51%).

Using impervious gowns by operating room staff reduces the risk of infection. By wearing a protective footwear, the medical personnel prevents feet pollution by bodily fluids. During an operation, the surgeon may use cautery or hand diathermy instead of a scalpel to perform skin incision or cut other tissues in the operating field. Avoiding hand-to-hand passage of sharps, particularly scalpels, is easily done by always placing them in a receiver such as a kidney dish. The most important is the choice of an experienced surgeon to perform the operation. In practice, this may do more than any other precaution to control the spread of the patient’s body fluids and tissues and therefore pathogens. All needles and sharps tools after use shall be disposed in a plastic container with thick walls, which has only a small inlet opening at the top. The container should be disposed of when is full at two-thirds capacity.

Postexposure prophylaxis

The most significant form of prevention of HIV infection among medical staff is to prevent exposure to blood or other body fluids. The term “exposure to HIV” means contact of mucous membrane or damaged skin surface (ie, minor skin injuries and inflammations) or percutaneous injury with potentially infectious body fluids.

Despite precaution, if the surgeon, anesthetist, or any other health care employee comes to exposure of HIV infection, postexposure prophylaxis (PEP), which is to take antiviral drugs as quickly as possible, will be effective. The antiviral drugs should be taken maximum of 72 hours after the exposure and at least for 4 weeks.The purpose of these drugs is to stop the replication of the virus, which is to prevent a carrier of the virus. However, the PEP may not be always effective. To increase PEP effectiveness in practice, we must apply the proper workflow. In the first step, we should examine the patient toward his or her viremic status and carefully estimate to what they have been exposed to (dirt conjunctivitis, needlestick, or others). Depending on the nature of the inoculum and the patient’s viremic status, we can start the pharmacological treatment (usually consisting of three drugs) as soon as possible. The best solution is the ability to consult with an expert on PEP, which selects the appropriate regimen of drugs depending on the type of exposure, medical history, drug interactions, and possible drug resistance. Currently, there are six classes of antiretroviral drugs, but usually in PEP, nucleoside reverse transcriptase inhibitors, integrase inhibitors, protease inhibitors, and non-nucleoside reverse transcriptase inhibitors are used. According to the latest recommendations, the PEP usually begins with the combination of three antiretroviral drugs (or even four if there are indications) and lasts for 4 weeks.The implementation of new therapies in the treatment of AIDS and patients with HIV is much more effective. Combination therapy containing HIV protease inhibitors is often determined as highly effective antiretroviral therapy. All health care workers (patients) receiving a PEP should complete a 4-week treatment. Unfortunately, according to data published in the literature, many health care employees who started PEP with old generation of antiretroviral drugs did not take a full 4-week treatment regimen because of numerous side effects. All the antiretroviral drugs cause side effects, for example, vomiting, rash, abdominal pain, diarrhea, hyperbilirubinemia, jaundice, fever, headaches, liver damage, nephrolithiasis, dizziness, sleep disturbances, respiratory symptoms, and others; therefore, proper selection of antiretroviral drugs according to their toxicity and the ability to easily reverse the side effects is a key in choosing a regimen for PEP. Therefore, a detailed discussion with the person who is going to start prevention and the use of drugs that reduce side effects helps to complete a full 4-week treatment regimen by a person who has been exposed to HIV infection. PEP can be stopped only in a situation where it is proved that the infectious material comes from HIV-negative patient, which is not in “serological window”. Another problem is with the pregnant health care workers who are exposed to HIV. According to the guidelines, in this situation, the postexposure procedure is the same as with any other person who has had exposure to the HIV. The decision to begin postexposure treatment in pregnancy must be carefully discussed between the woman and the doctor on the potential benefits and side effects for both the mother and the fetus in association with the use of antiretroviral drugs. We already know that we should avoid certain drugs during pregnancy, for example, efavirenz, stavudine, didanosine, and indinavir because they are teratogenic and may cause lactic acidosis or hyperbilirubinemia in newborns. A regimen containing three (or more) antiretroviral drugs is now recommended routinely for all occupational exposures to HIV. Routinely, a regimen consisting of three drugs such as integrase inhibitors with nucleoside reverse transcriptase inhibitors, and a nonnucleoside inhibitor of reverse transcriptase or protease inhibitor is usually preferred.The recommended PEP regimen is emtricitabine with tenofovir, which can be taken as a single tablet (Truvada) once a day supplemented with raltegravir 400 mg twice daily. It is also a regimen that can be used in a pregnant woman. Due to the toxicity of tenofovir in patients with kidney disease, the drug may be replaced, for example, with zidovudine, which can be combined with lamivudine in the form of one drug (Combivir).

Health care workers who are exposed to HIV infection must be tested immediately in the direction of viremic status. Further tests are performed at 6 and 12 weeks and 4 months after exposure (duration of follow-up for exposed health care workers has been reduced to 4 months with the availability of the fourth-generation HIV tests – editor’s note). Extended observation is recommended for health care employees who are exposed to HCV infection from a patient who is a carrier of both HIV and HCV. Each patient receiving PEP must be monitored in the context of toxicity of antiretroviral drugs at baseline and after 2-week therapy. The minimum parameters that should be evaluated during therapy include blood count and kidney and liver function tests. Depending on the detected disorders, the treatment can be modified.