Why are the following not needed in a PCR reaction while they are needed for DNA...

Why are the following not needed in a PCR reaction while they are needed for DNA replication in a cell?

Helicase

Topoisomerase

Primase

rNTPs

RNase

DNA ligase

Solutions

Expert Solution

Firstly these all are enzyme and cannot actively work at higher temperature than body temperature.

Helicase generally unwinds the DNA strand, in PCR higher temperature (94ﹾC) or above unwinds and open the DNA strand.

topoisomerase used to cut the DNA strand release the tension of DNA strand. But again this work is performed by higher temperature govern step in PCR

Primase generally provides short sequences as a primer for starting replication. however incase of PCR this step is govern by ready to use sequence complimentary primers (which are generally 20 nucleotide long) and this step is also govern by temperature, called as annealing temperature, which helps primer to anneal with complementary sequence.

rNTPs helps in synthesis of primer in replication, but in PCR this process is done by dNTPs which again are ready to use.

RNase helps in catalyzing the degradation RNA molecules, in PCR this step is done by nuclease free water.

DNA ligase generally works as joins or ligates DNA molecules which are done by DNA polymerase in PCR.

gladiator answered 1 year ago

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