You are setting up a PCR reaction using DNA from a bacteria with a genome size...

You are setting up a PCR reaction using DNA from a bacteria with a genome size of 17 megabase-pairs (mb). How many nanograms of DNA will you need to add to the reaction to ensure there are at least 100 copies of the single gene you are trying to amplify? (assume the average molecular weight of a nucleotide pair is 650). (2 pt)

Expert Solution

PCR or Polymerase chain reaction is basically used to amplify the DNA copies. If we ought to give DNA, the requires environment like temparature and nucleotides, ligase,primer etc.. they can replicate and amplify. The total number of DNA we get from a certain amount of mix can be determined by the equation;

Number of copies of DNA = (DNA amount (ng) x 6.022x1023) / (length of DNA x 1x109ng/ml x 650 Daltons).

• Average weight of a single base pair is 650 Da.
• so number of base pair multiply to 650 gives the total weight of the DNA sequence we want to amplify. To make it in nanograms, multiply by 10^9.
• Amount of DNA taken in nanogram multiplied by avagadro number gives the total DNA.
• Avagadro number- 6.022×10^23

Thus the calculation is,

The amount of DNA in ng needed to get 100 copies of a sequence which extend 17 mbp is 0.00183 nanograms.

gladiator answered 2 years ago

Explain how PCR (polymerase chain reaction) works. After explaining PCR, compare PCR to the DNA replication...

Explain how PCR (polymerase chain reaction) works. After explaining PCR, compare PCR to the DNA replication that occurs naturally in living cells, making sure to give at least 4 similarities and 4 differences.

what does the polymerase chain reaction (PCR) accomplish? 1) PCR converts RNA to DNA 2) PCR...

what does the polymerase chain reaction (PCR) accomplish? 1) PCR converts RNA to DNA 2) PCR corrects mutation in a gene 3) PCR makes many copies of DNA segment 4) PCR joins short segments of DNA to make one long segment how does the ribosome select the next amino acid to add to a growing polypeptide chain? 1) the ribosome selects the amino acid that can form hydrogen bonds with the mRNA 2) the ribosome selects a tRNA that base-pairs...

Why are the following not needed in a PCR reaction while they are needed for DNA...

Why are the following not needed in a PCR reaction while they are needed for DNA replication in a cell? Helicase Topoisomerase Primase rNTPs RNase DNA ligase

You are interested in cloning a gene from the B. sanfranciscus genome, so you design PCR...

You are interested in cloning a gene from the B. sanfranciscus genome, so you design PCR primers that should amplify a 1 kilobase pair (kbp) PCR product that contains the gene of interest. After amplification, you will see if the PCR was successful by loading the entire reaction onto an agarose gel and performing electrophoresis to see if a product of the expected size was generated. To visualize the DNA, you will stain the gel with a fluorescent dye called ethidium bromide, which...

7.You want to set up a PCR reaction. The protocol suggests a 50 µL reaction volume...

7.You want to set up a PCR reaction. The protocol suggests a 50 µL reaction volume containing 1X buffer, 200 µM dNTPs, 0.5 µM each primer (forward and reverse), 250 ng template DNA, and 1 U enzyme. You have the following reagents: 5 X buffer, 10 mM dNTPs, 10 µM each primer (forward and reverse), 125 ng/µL template DNA, and 2 U/µL enzyme. How many µL of water, reaction buffer, dNTPs, each primer, template DNA and enzyme would you combine...

When setting up a PCR experiment, you must prepare a positive control sample. What would the...

When setting up a PCR experiment, you must prepare a positive control sample. What would the positive control help you discover? A) You forgot to add Loading Dye to your samples B) The DNA ladder is denatured C) Your thermocycler is broken D) Your reagents are contaminated with foreign DNA

Can DNA be amplified from a degraded sample for PCR?

If you would do a PCR experiment using the Klenow fragment of the E.coli DNA polymerase...

If you would do a PCR experiment using the Klenow fragment of the E.coli DNA polymerase I instead of Taq DNA polymerase, what would you have to change in the experimental set-up and explain why?

in pcr practical, Briefly describe the reaction mechanism leading to the synthesis of a new DNA...

in pcr practical, Briefly describe the reaction mechanism leading to the synthesis of a new DNA strand by Taq DNA polymerase. In your answer include: i. The mechanism of the synthesis reaction, ii. The direction of the synthesis reaction iii. The direction in which sequence information is read from the template strand.

PCR reactions are specific to particular pieces of DNA. Which component of the reaction allows for...

PCR reactions are specific to particular pieces of DNA. Which component of the reaction allows for this specificity? Explain how. Sanger Sequencing (also called chain termination and dideoxy-sequencing) utilizes special dideoxy nucleotides (ddNTPs). What is the difference between these special nucleotides and the dNTPs used in PCR? What role do the ddNTPs play in the sequencing reaction?

Question