You are setting up a PCR reaction using DNA from a bacteria with a genome size...

You are setting up a PCR reaction using DNA from a bacteria with a genome size of 17 megabase-pairs (mb). How many nanograms of DNA will you need to add to the reaction to ensure there are at least 100 copies of the single gene you are trying to amplify? (assume the average molecular weight of a nucleotide pair is 650). (2 pt)

Expert Solution

PCR or Polymerase chain reaction is basically used to amplify the DNA copies. If we ought to give DNA, the requires environment like temparature and nucleotides, ligase,primer etc.. they can replicate and amplify. The total number of DNA we get from a certain amount of mix can be determined by the equation;

Number of copies of DNA = (DNA amount (ng) x 6.022x1023) / (length of DNA x 1x109ng/ml x 650 Daltons).

• Average weight of a single base pair is 650 Da.
• so number of base pair multiply to 650 gives the total weight of the DNA sequence we want to amplify. To make it in nanograms, multiply by 10^9.
• Amount of DNA taken in nanogram multiplied by avagadro number gives the total DNA.
• Avagadro number- 6.022×10^23

Thus the calculation is,

The amount of DNA in ng needed to get 100 copies of a sequence which extend 17 mbp is 0.00183 nanograms.

gladiator answered 2 years ago

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