In CE utilizing photometric (UV) detection, it is often found to be difficult because of inadequate...

In CE utilizing photometric (UV) detection, it is often found to be difficult because of inadequate detector sensitivity.

a) why is UV detection of low sensitivity?

b) list all remedy(s) that can be had here.

Expert Solution

a)

Although capillary electrophoresis (CE) as a separation method has the advantages of rapid separation and high resolution, one of limitations is its detection capabilities. Absorbance, fluorescence, electrochemical, and refractive index have been the major detection modalities of CE. Among them, on-column absorbance detection has been most commonly used for CE. The short path length inherent in capillaries of I.D. less than 100 µm, however, limits the detection sensitivity that is achieved with absorbance detection.

B)

1. Several attempts have been made to improve the detection sensitivity by extending the optical path length.

2.To overcome this limitation and to increase the sensitivity, two strategies are applied: first, to increase the amount of analyte added to the capillary.

3. increase the sensitivity on the detector site. To improve the sensitivity on the detector site, alternative detection techniques to UV detection, e.g., laser-induced fluorescence detection (LIF) or mass spectroscopy (MS), can be applied.

queen_honey_blossom answered 2 years ago

4. The practical value of using UV absorbance as a method of detection is that: A....

4. The practical value of using UV absorbance as a method of detection is that: A. Any given compound can be detected by UV absorption, so it is a universal detection technique. B. It can be used to correlate a measurable quantity (absorbance) to a quantity that cannot be measured directly (concentration), according to Beer’s Law. C. Neither A nor B are correct. D. Both A and B are correct.

In healthcare industry, it is difficult to get accurate incident statistics because violence is often underreported....

In healthcare industry, it is difficult to get accurate incident statistics because violence is often underreported. What would an employee fail to report a threatening or injurious action, directly affecting his safety n wellbeing? There are concerns that assault by pts n visitor may be viewed as result of poor performance of=r negligence of employee. There is lack of institutional reporting policies; that is, employee doesn’t know how to report it Some employees have perception that assault are part of...

a) The marginalists found that the classical theory of value was inadequate to explain the forces...

a) The marginalists found that the classical theory of value was inadequate to explain the forces determining prices. Value does not come from the past but value comes from the future. Factors of production are not price-determining but price-determined. Discuss ( 30 marks) b) The study of an economy is complex and difficult. How did Marshall address this problem? How did he reconcile the positions of the classical and marginal Wily schools?

Standing still can cause a person to faint because of inadequate return of blood to the...

Standing still can cause a person to faint because of inadequate return of blood to the heart via veins. Which of the following is NOT occurring to promote return of bland. ventricular contractio or skeletal muscle activily compressing veins and forcing blood flow

What makes early detection of an ovarian tumor so difficult? Are there any steps that can...

What makes early detection of an ovarian tumor so difficult? Are there any steps that can be taken in order to prevent or reduce the chances of developing an ovarian tumor?

Why is a V vs S plot often inadequate for determining enzyme kinetic constants, and how...

Why is a V vs S plot often inadequate for determining enzyme kinetic constants, and how do various alternate plotting methods allow these values to be determined more adequately? Give examples of alternate methods of kinetic analysis and their utility. The V vs S plot is inadequate because it makes the assumption that it’s only for a single subunit and substrate. The line on the graph usually represents a line that best fits the data and are not completely accurate....

If, according New Classical economists, fiscal policy is inadequate because any government spending increase causes: a....

If, according New Classical economists, fiscal policy is inadequate because any government spending increase causes: a. most households save rather than spend. b. business to decrease their investments. c. private individuals to spend more and save less.

Because accounting is considered the language of business, it may be difficult to think there is...

Because accounting is considered the language of business, it may be difficult to think there is a relationship between accounting and faith; however, the Christian worldview should and can permeate every discipline, even accounting. In what way(s) do you think the Christian worldview can integrate with accounting?

In humans, lighter skin color is adaptive at latitudes far from the equator because UV light...

In humans, lighter skin color is adaptive at latitudes far from the equator because UV light  stimulates synthesis of folic acid  causes breakdown of folic acid  stimulates synthesis of vitamin D  causes breakdown of vitamin D

It is often difficult to find the exact mean average of a large population of data....

It is often difficult to find the exact mean average of a large population of data. We use confidence Intervals to estimate where we think the true mean lies. Discuss the advantages of using a confidence interval. Give an example of a large population of data that is suited to a confidence interval estimate- in other words, it would be very hard to find the exact true mean of the data.

Question