4. The practical value of using UV absorbance as a method of detection is that: A....

4. The practical value of using UV absorbance as a method of detection is that:

A. Any given compound can be detected by UV absorption, so it is a universal detection technique.
B. It can be used to correlate a measurable quantity (absorbance) to a quantity that cannot be measured directly (concentration), according to Beer’s Law.
C. Neither A nor B are correct.
D. Both A and B are correct.

Expert Solution

A. Any given compound can be detected by UV absorption, so it is a universal detection technique.

All molecules do not give absorbance in the UV range. Hence, UV absorptions are not universal detection techniques. Therefore, this option is not correct.

B. It can be used to correlate a measurable quantity (absorbance) to a quantity that cannot be measured directly (concentration), according to Beer’s Law.
According to Beer-Lambert Law, there is a linear relationship between absorbance and the concentration of absorbing compound. Therefore, the above statement is correct.
Hence, this option is correct.

C. Neither A nor B are correct.
From, the above, the statement B is correct. Hence, the option is C is not correct.

D. Both A and B are correct.
Obviously, this option is not correct.

Hence, the correct answer is (B); It can be used to correlate a measurable quantity (absorbance) to a quantity that cannot be measured directly (concentration), according to Beer’s Law.

queen_honey_blossom answered 1 year ago

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