Question

It is described that for a human protein that is expressed only in the brain, There is an identical but smaller variant that is expressed only in the liver. What experiments would you use to determine the origin of this differential expression?

Answer 1

- Yes, we all know brain and liver both are complex organ that controls a variety of bodily fuctions. Brain : It perfomed fuction like maintenance of homeostasis, processing of sensory information and genration of behaviour.Liver : production of bile ,hormone and vitamins, storage of glycogen and removal of toxic substsnce.

The human genome projects revealed the gentic code ,enabling extensive analysis of gene expression in tissue and organ samples in the context of evolution, physiology and disease. The transcriptomes of 26 pheripheral human organs and three fronatal cortex samples were analyzed using next generation sequencing based on specimens. The transcriptome of each sample was quantified using RNAseq to determine the normalized mRNA abundance.High correlation between biological replicates indicates low inter- individual variability in gene expression.

Transcriptome analysis of the liver can be visualized with regard to specificity and distribution of transcribed mRNA molecules. Specificity illustrate teh number of gene with elevated or non elevated expression in the liver compared to other tissues.In depth analysis of elevated genes in liver using antibody based protein profiling allowed us to visualize the expression patterns of these proteins in different fuctional compartments including plasma proteins, enzymes, bile proteins and transporters.