Which components of a microscope are needed to provide the
necessary magnification, resolution, and contrast? Why?...
Which components of a microscope are needed to provide the
necessary magnification, resolution, and contrast? Why?
thanks
Solutions
Expert Solution
The eyepiece - It contains the ocular lens,
through which the user looks to see the magnified specimen. The
ocular lens has a magnification that can range from 5x to 20x.
Objective turret - A revolving nose piece to
adjust the magnification power is the part that holds the set of
objective lenses.
The objective lenses - They combine with the
eyepiece lens to increase magnification levels. Microscopes
generally feature three or four objective lenses, with
magnification levels ranging 4x to 100x.
The condenser lens - It works with the
diaphragm to focus the intensity of the light source onto the slide
containing the specimen.
Focus knobs - Adjustment knobs move the stage
up and down with separate adjustment for coarse and fine
focusing.
Stage - A mechanical stage allows tiny
movements of the slide via control knobs that reposition the
sample/slide as desired. All stages move up and down for focus.
Moving to a higher magnification requires the stage to be moved
higher vertically for re-focus at the higher magnification and may
also require slight horizontal specimen position adjustment.
The light source - Microscopes require a light
source for viewing. A built-in, low-voltage illuminator light, or a
mirror that reflects an external light source like sunlight are
used.
The microscope that gives the greatest
resolution, and therefore the highest useful magnification is
the:
A. compound light
microscope;
B. transmission electron
microscope;
C. darkfield
microscope;
D. scanning electron
microscope;
E. fluorescence microscope.
By
considering the maximum resolution possible, estimate the minimum
useful magnification of the optical microscope and the
scanning electron microscope.
define the following of a microscope
(a) Magnification
(b) resolution
(c) depth of field
(d) field of view
(2) what are the differences between a stereo microscope and a
compound microscope
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1) which light source will provide you with greater resolution
green, blue or red light?
2) whch objective is most useful to resolve a mitochondria
(apprx, size = 750 nm)?
3) how does the image differ in terms of orientation from the
unmagnified image?
4) since the depth of field only allows you to focus a limited
number of focal planes at a time; will you be able to tell what is
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