Question

Cell Biology : Microscope

1) which light source will provide you with greater resolution green, blue or red light?

2) whch objective is most useful to resolve a mitochondria (apprx, size = 750 nm)?

3) how does the image differ in terms of orientation from the unmagnified image?

4) since the depth of field only allows you to focus a limited number of focal planes at a time; will you be able to tell what is above or below in your biological sample? If no, why not? If yes, how?

Answer 1

please find the answer of first two part only(1,2)

Question 1 which light source will provide you with greater resolution green, blue or red light

Answer is blue ,green and red.

1. The wavelength of light is an important factor in the resolution of a microscope.
2. Shorter wavelengths give up higher resolution and visa versa. The greatest resolving power in optical microscopy is realized with near-ultraviolet light, the shortest effective imaging wavelength.
3. Near-ultraviolet light is followed by blue, then green, and finally red light in the ability to resolve specimen detail.
4. Visible light has a wavelength range of about 400 nm to 750 nm. The visible colours from shortest to longest wavelength are: violet, indigo, blue, cyan,green, yellow, orange, and red

Question 2 whch objective is most useful to resolve a mitochondria (apprx, size = 750 nm)?

1. Microscopes have been play an important role for our understanding of mitochondrial structure and function.
2. Mitochondria are visible under the light microscope although little detail can be seen.
3. Transmission electron microscopy shows the complex internal membrane structure of mitochondria, and electron tomography gives a three-dimensional view.