Question

Describe the following 3 types of chromatography: thin-layer chromatography, flash column chromatography, and gas chromatography. For each type describe the scale (how much compound can be used), the utility and describe the 2 phases that correspond with that type of chromatography.

2. what is necessary for separation using chromatography

3. what is normal phase and reverse phase chromatography

if you could link me a video so i could learn I will add points.

Answer 1

Gas Chromatography

Liquid Chromatography

Ion Exchange Chromatography

Affinity Chromatography

The Different Types of Chromatography

There are four main types of chromatography. These are Liquid Chromatography, Gas Chromatography, Thin-Layer Chromatography and Paper Chromatography.

Liquid Chromatography is used in the world to test water samples to look for pollution in lakes and rivers. It is used to analyze metal ions and organic compounds in solutions. Liquid chromatography uses liquids which may incorporate hydrophilic, insoluble molecules.

Gas Chromatography is used in airports to detect bombs and is used is forensics in many different ways. It is used to analyze fibers on a persons body and also analyze blood found at a crime scene. In gas chromatography helium is used to move a gaseous mixture through a column of absorbent material.

Thin-layer Chromatography uses an absorbent material on flat glass or plastic plates. This is a simple and rapid method to check the purity of an organic compound. It is used to detect pesticide or insecticide residues in food. Thin-layer chromatography is also used in forensics to analyze the dye composition of fibers.

Paper Chromatography is one of the most common types of chromatography. It uses a strip of paper as the stationary phase. Capillary action is used to pull the solvents up through the paper and separate the solutes.