Question

When would the application of thin-layer chromatography, adsorption column chromatography, and gas chromatography be appropriate to use?

Answer 1

thin layer chromatography (TLC) can not only separate the components from a mixture easily, but can also help you determine the identity of the compounds (by using developing reagents and Rf values) within a brief period of time. Detection of a Particular Compound Present in a Mixture the number of spots developed on the TLC plate to approximate the number of compounds in the mixture.

Column chromatography is one of the most useful methods for the separation and purification of both solids and liquids. This is a solid - liquid technique in which the stationary phase is a solid & mobile phase is a liquid. The principle of column chromatography is based on differential adsorption of substance by the adsorbent. This method is very useful when the components of a mixture have almost the same physical and chemical properties and hence can’t be separated by other usual methods of separations

Gas chromatography has the capacity to resolve the components of complex mixtures of compounds such as petroleum fractions or smoke which can contain hundreds or even more components in a single sample Such complex resolutions are virtually impossible using TLC techniques. Gas chromatography is ideally suited for analysis of volatile compounds. Samples with boiling points as high as 380 to 400°C can be analysed by temperature programming. The samples can range from liquids, gases and dissolved solids. TLC techniques, however, cannot be used for analysis of gases.