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In: Chemistry

Compare and contrast Column (solid-liquid) chromatography, Paper Chromatography, and thin layer chromatography in respect to the...

Compare and contrast Column (solid-liquid) chromatography, Paper Chromatography, and thin layer chromatography in respect to the size of the sample that can be separated, the ease of use, and the versatility of the method.

Solutions

Expert Solution

ADSORPTION CHROMATOGRAPHY:   

1) COLUMN CHROMATOGRAPHY : large scale seperations , mobile phase : liquid , stationary phase : solid

quantity of the sample needed is high because the sample has to be place in a column for seperations.

2) THIN LAYER CHROMATOGRAPHY : qualitative analysis (identification of organic compounds ) ,

mobile phase : liquid , stationary phase : solid (silica gel and alumina ) quantity of the sample needed is small because the sample has to be placed on a layer of adsorbent .

gives better result as compre to paper chromatography bands form are sharper

PARTITION CHROMATOGRAPHY :

1)PAPER CHROMATOGRAPHY : qualitative and quantitative (polar organic compounds and inorganic compounds )

    mobile phase : liquid , stationary phase : liquid

quantity of the sample needed is small because the sample has to be placed on a thin layer of chromatogram


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