Summarize the procedure of Thin Layer Chromatography (3-4 sentences)

Expert Solution

With a pencil, a thin mark is made at the bottom of the plate to apply the sample spots.
Then, samples solutions are applied on the spots marked on the line in equal distances.
The mobile phase is poured into the TLC chamber to a leveled few centimeters above the chamber bottom. A moistened filter paper in mobile phase is placed on the inner wall of the chamber to maintain equal humidity (and also thereby avoids edge effect this way).
Now, the plate prepared with sample spotting is placed in TLC chamber so that the side of the plate with the sample line is facing the mobile phase. Then the chamber is closed with a lid.
The plate is then immersed, such that the sample spots are well above the level of mobile phase (but not immersed in the solvent — as shown in the picture) for development.
Allow sufficient time for the development of spots. Then remove the plates and allow them to dry. The sample spots can now be seen in a suitable UV light chamber, or any other methods as recommended for the said sample.

queen_honey_blossom answered 2 years ago

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