Question

In: Chemistry

Describe how multi-column chromatography can be advantageous over single column chromatography. Include the following terms in...

Describe how multi-column chromatography can be advantageous over single column chromatography. Include the following terms in your explanation: binding capacity, breakthrough

Solutions

Expert Solution


Related Solutions

elect all statements below that apply to Column Chromatography. Column chromatography can be used to purify...
elect all statements below that apply to Column Chromatography. Column chromatography can be used to purify small (< 0.1 g) sample mixtures. The compound in a mixture with the lowest Rf value will exit the column first. Column chromatography is often used to purify large (10 g) sample mixtures. Column chromatography is used mainly as an analytical tool.
The following data apply to apply to a column for liquid chromatography:                    Length of column:...
The following data apply to apply to a column for liquid chromatography:                    Length of column: 25.7 cm                    Flow rate:            0.313 mL/min                    VM:                      1.37 mL                    VS:                       0.164 mL A chromatogram of species A and B provided the following data: Retention Time, min Width of Peak Base (W), min Non-retained 3.1 - A 13.3 1.07 B 14.1 1.16 a)    the expected resolution, if the column length was doubled b)    the expected capacity factors,...
Explain how column chromatography can be used to separate a mixture on a larger scale than...
Explain how column chromatography can be used to separate a mixture on a larger scale than TLC?
compare column, thin-layer, and gas chromatography in terms of mobile phase and stationary phase
compare column, thin-layer, and gas chromatography in terms of mobile phase and stationary phase
Describe the principle of separation in a column used for size – exclusion chromatography. What is...
Describe the principle of separation in a column used for size – exclusion chromatography. What is the separation range in term of molecular weight? Give the definition of “gel filtration: and “gel permeation”.   
Match the following terms in column 1 with their description or definition in column 2. Column...
Match the following terms in column 1 with their description or definition in column 2. Column 1. Column 2 Mitral Valve.                   the right atrioventricular valve Semilunar Valve.            the right semilunar valve Tricuspid Valve              the left semilunar valve Aortic Valve.                   the heart valves between the                                           atria and great veins                                           heart valve between ventricle                                           and great arteries                                           the left atrioventricular Valve
17. Which of the following credit terms is the most advantageous to the purchaser of merchandise?...
17. Which of the following credit terms is the most advantageous to the purchaser of merchandise? a. 1/10, n30. b. 4/40, n120. c. 2/10, n30. d. 2/5, n15. 18. Which of the following is always included in the current assets section of the classified balance sheet of a merchandiser? a. Cash b. Trade Accounts Receivable c. Prepaid Rent d. All of the above 19. Which of the following is not one of the transactions that comprise the operating cycle of...
Explain how liquids can be analyzed by gas chromatography. Briefly define or describe the function, in...
Explain how liquids can be analyzed by gas chromatography. Briefly define or describe the function, in gas chromatography, of the carrier gas: stationary liquid phase: solid support: If ethyl acetate and n-butyl acetate are analyzed by gas chromatography, which of these esters will generally produce a peak with the shorter retention time? What operating variables determine retention time? Explain why is it important to inject the sample quickly.
" ion chromatography " is a nice self -contained experiment . 1- how do suppressor column...
" ion chromatography " is a nice self -contained experiment . 1- how do suppressor column work ?2- discuss some applications of this technique ? 3) why analyze soils with ion chromatography?
1. Describe DNA synthesis and describe why Okazaki fragments are needed? include the following terms in...
1. Describe DNA synthesis and describe why Okazaki fragments are needed? include the following terms in any order (and please underline them in your answer): leading strand, lagging strand, replication fork, DNA polymerase, direction of DNA synthesis, origin of replication, primer, ligase.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT