Describe how multi-column chromatography can be advantageous
over single column chromatography. Include the following terms in...
Describe how multi-column chromatography can be advantageous
over single column chromatography. Include the following terms in
your explanation: binding capacity, breakthrough
elect all statements below that apply to Column
Chromatography.
Column chromatography can be used to purify small (< 0.1 g)
sample mixtures.
The compound in a mixture with the lowest Rf value will exit the
column first.
Column chromatography is often used to purify large (10 g)
sample mixtures.
Column chromatography is used mainly as an analytical tool.
The following data apply to apply to a
column for liquid chromatography:
Length of column: 25.7 cm
Flow rate:
0.313 mL/min
VM:
1.37 mL
VS:
0.164 mL
A chromatogram of species A and B
provided the following data:
Retention Time, min
Width of Peak Base (W), min
Non-retained
3.1
-
A
13.3
1.07
B
14.1
1.16
a) the expected resolution, if the column length was
doubled
b) the expected capacity factors,...
Describe the principle of separation in a column used for size –
exclusion chromatography. What is the separation range in term of
molecular weight? Give the definition of “gel filtration: and “gel
permeation”.
Match the following terms in column 1 with their description or
definition in column 2.
Column 1. Column 2
Mitral
Valve.
the right atrioventricular valve
Semilunar
Valve.
the right semilunar valve
Tricuspid
Valve
the left semilunar valve
Aortic
Valve.
the heart valves between the
atria and great veins
heart valve between ventricle
and great arteries
the left atrioventricular Valve
17. Which of the following credit terms is the most advantageous
to the purchaser of merchandise?
a. 1/10, n30.
b. 4/40, n120.
c. 2/10, n30.
d. 2/5, n15.
18. Which of the following is always included in the current
assets section of the classified balance sheet of a
merchandiser?
a. Cash
b. Trade Accounts Receivable
c. Prepaid Rent
d. All of the above
19. Which of the following is not one of the transactions that
comprise the operating cycle of...
Explain how liquids can be analyzed by gas chromatography.
Briefly define or describe the function, in gas chromatography,
of the
carrier gas:
stationary liquid phase:
solid support:
If ethyl acetate and n-butyl acetate are analyzed by gas
chromatography, which of these esters will generally produce a peak
with the shorter retention time?
What operating variables determine retention time?
Explain why is it important to inject the sample quickly.
" ion chromatography " is a nice self -contained experiment . 1-
how do suppressor column work ?2- discuss some applications of this
technique ? 3) why analyze soils with ion chromatography?
1. Describe DNA synthesis and describe why Okazaki fragments are
needed? include the following terms in any order (and please
underline them in your answer): leading strand, lagging strand,
replication fork, DNA polymerase, direction of DNA synthesis,
origin of replication, primer, ligase.