How is thin layer chromatography technique of separation similar to a paper chromatographic separation?

Expert Solution

	Thin Layer Chromatography	Paper chromatography
Basic application	Thin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures.	Paper chromatography is an analytical method that is used to separate coloured chemicals or substances.This can also be used in secondary or primary colours in ink experiments. This method has been largely replaced by thin layer chromatography, but is still a powerful teaching tool. It is type of TLC.
Solid support and phases	Thin-layer chromatography is performed on a sheet of glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide, or cellulose. This layer of adsorbent is known as the stationary phase.	The stationary phase is usually a piece of high quality filter paper. The mobile phase is a developing solution that travels up the stationary phase, carrying the samples with it. Components of the sample will separate readily according to how strongly they adsorb onto the stationary phase versus how readily they dissolve in the mobile phase.
Principle of working:same	Separation of compounds is based on the competition of the solute and the mobile phase for binding places on the stationary phase.^[3] For instance, if normal-phase silica gel is used as the stationary phase, it can be considered polar. Given two compounds that differ in polarity, the more polar compound has a stronger interaction with the silica and is, therefore, more capable to dispel the mobile phase from the binding places.	Separations in paper chromatography involve the same principles as those in thin layer chromatography, as it is a type of thin layer chromatography.

Both Thin Layer Chromatography and Paper chromatography are used for separation of compounds.

They have certain similarities and differences in between; I tried to make it in tabular format that will be easy for comparison-

queen_honey_blossom answered 2 years ago

Give two advantages of thin layer chromatography over paper chromatography.

Compare and contrast Column (solid-liquid) chromatography, Paper Chromatography, and thin layer chromatography in respect to the...

Compare and contrast Column (solid-liquid) chromatography, Paper Chromatography, and thin layer chromatography in respect to the size of the sample that can be separated, the ease of use, and the versatility of the method.

Thin-layer chromatography is a relatively low tech chromatographic method (no expensive instrumentation is required). What are...

Thin-layer chromatography is a relatively low tech chromatographic method (no expensive instrumentation is required). What are the potential advantages of this method over gas chromatography or high pressure liquid chromatography?

Experiment 7: Thin Layer Chromatography What is the name of the technique you will be using...

Experiment 7: Thin Layer Chromatography What is the name of the technique you will be using in experiment No.7 lab? Thin Layer Chromatography Mass Spectrometry Thick Liquid Calorimetry Liquid Chamber Chromatography TLC plates separate molecules on the basis of: Polarity Color Size Bond orientation The mixture is dissolved in a fluid called the ___________ phase, which carries it through a structure holding another material called the __________ phase. The polarity of a bond between two elements can be best determined...

Thin Layer Chromatography utilizes a glass or metal plate coated with a thin layer of absorbent...

Thin Layer Chromatography utilizes a glass or metal plate coated with a thin layer of absorbent material such as silica gel. The sample is prepared similiarto paper chromatography and a solvent is used to elute the sample. 1. Which is the stationary phase? Plate Silica Gel Solvent 2. Which is the mobile phase? Plate Silica Gel Solvent 3. Which component is inert? Plate Silica Gel Solvent

How pigment separation using paper chromatography works?

Explain and compare thin layer chromatography with column chromatography (in-detailed answer)

1.) What factors affect Rf values in thin-layer chromatography? 2.) What factors affect the separation on...

1.) What factors affect Rf values in thin-layer chromatography? 2.) What factors affect the separation on the column in column chromatography? 3.) What factors affect the resolution of the substances and their retention times in gas chromatography?

When would the application of thin-layer chromatography, adsorption column chromatography, and gas chromatography be appropriate to...

When would the application of thin-layer chromatography, adsorption column chromatography, and gas chromatography be appropriate to use?

Set up a table and compare and contrast these 4-chromatographic/separation techniques (Gas Chromatography, Liquid Chromatography, Electrophoresis,...

Set up a table and compare and contrast these 4-chromatographic/separation techniques (Gas Chromatography, Liquid Chromatography, Electrophoresis, Size Exclusion Chromatography). Make sure to include what characteristic the separation is based on, the mobile phase, the stationary phases, and an available detection method for each technique. Thank you!

Question