You have added an irreversible inhibitor to a sample of enzyme and substrate. At this point, the reaction has stopped completely. What can you do to regain the activity of the enzyme?
4. The enzyme is inactive at this point. A new enzyme must be added to regain enzyme activity
1. The inhibitor is irreversible. So not possible
2. When the substrate is added proportionate amount of enzyme will not be there for the reaction
3. Not sensible