In: Biology
If the substrate concentration is limiting, how does increasing enzyme concentration affect reaction rate?
A.Increasing enzyme concentration has no effect on reaction rate.
B.Increasing enzyme concentration results in an increase in reaction rate. C.Increasing enzyme concentration decreases reaction rate. |
Answer:
A. Increasing enzyme concentration has no effect on reaction rate.
Changing the Enzyme and Substrate concentrations affect the rate of reaction of an enzyme-catalysed reaction. If it is the limiting factor, increasing concentration will increase the rate of reaction up to a point, after which any increase will not affect the rate of reaction. This is because it will no longer be the limiting factor and another factor will be limiting the maximum rate of reaction. As a reaction proceeds, the rate of reaction will decrease, since the Substrate will get used up. After a certain concentration, any increase will have no effect on the rate of reaction, since Substrate Concentration will no longer be the limiting factor. Thus any further increase in enzyme concentration has no further effect on the reaction rate.