Question

In: Biology

If the substrate concentration is limiting, how does increasing enzyme concentration affect reaction rate? A.Increasing enzyme...

If the substrate concentration is limiting, how does increasing enzyme concentration affect reaction rate?

A.Increasing enzyme concentration has no effect on reaction rate.

B.Increasing enzyme concentration results in an increase in reaction rate.

C.Increasing enzyme concentration decreases reaction rate.

Solutions

Expert Solution

Answer:

A. Increasing enzyme concentration has no effect on reaction rate.

Changing the Enzyme and Substrate concentrations affect the rate of reaction of an enzyme-catalysed reaction. If it is the limiting factor, increasing concentration will increase the rate of reaction up to a point, after which any increase will not affect the rate of reaction. This is because it will no longer be the limiting factor and another factor will be limiting the maximum rate of reaction. As a reaction proceeds, the rate of reaction will decrease, since the Substrate will get used up. After a certain concentration, any increase will have no effect on the rate of reaction, since Substrate Concentration will no longer be the limiting factor. Thus any further increase in enzyme concentration has no further effect on the reaction rate.


Related Solutions

1a) what is the relationship between substrate concentration and reaction rate? b) Describe how the enzyme...
1a) what is the relationship between substrate concentration and reaction rate? b) Describe how the enzyme structure is effected: i) below the optimal temperature ii)above the optimal temperature?   c)Why is enzyme activity reduced at pH values above or below the optimum? How does the enzyme structure change at non-optimum pH?
How does temperature and pH affect the ability of an enzyme to bind substrate? and what...
How does temperature and pH affect the ability of an enzyme to bind substrate? and what specific changes occur to the structure of an enzyme when it is boiled?
1. (3%) You measure the initial rate of an enzyme reaction as a function of substrate...
1. (3%) You measure the initial rate of an enzyme reaction as a function of substrate concentration in the presence and absence of an inhibitor. The following data are obtained: [S] V0 –Inhibitor +Inhibitor 0.0001 33 17 0.0002 50 29 0.0005 71 50 0.001 83 67 0.002 91 80 0.005 96 91 0.01 98 95 0.02 99 98 0.05 100 99 0.1 100 100 0.2 100 100 a) What is the Vmax in the absence of inhibitor? b) What is...
3. What happens to the enzyme and the substrate during a chemical reaction? What does it...
3. What happens to the enzyme and the substrate during a chemical reaction? What does it mean by enzymes being “specific”? Why is the active site specific for only one type of substrate? Please type the answer. Thanks
Which of the following statements about enzyme substrate complexes is TRUE? A. At high substrate concentration...
Which of the following statements about enzyme substrate complexes is TRUE? A. At high substrate concentration the rate of the reaction is first order. B. The enzyme concentration is higher than the substrate concentration under physiological conditions. C. Alteration in the conformation of the enzyme may occur when substrate binds. D. Increase in enzyme concentration does not affect the rate of the reaction.
2) The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate...
2) The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are given below. [S](mu*M) 5 10 20 50 100 200 500 vo (mu*Mmin-1) 21 62 92 126 185 210 217 a) Estimate Vmax and Km from a direct graph of vo vs [S] (Michaelis-Menton plot). b) Use a Lineweaver-Burk plot to analyze the same data. What are the values of Vmax and Km? c) If the total enzyme concentration is 100 pM,...
Sulfanilamide that resembles substrate of an enzyme inhibits the enzyme when added to the reaction mix....
Sulfanilamide that resembles substrate of an enzyme inhibits the enzyme when added to the reaction mix. What type of inhibition is this A) allosteric inhibition B) competitive inhibition C) excitatory allosteric control D) noncompetitive inhibition E) feedback inhibition
1) How does increasing the degree of unsaturation affect the rate of the Sn1 and the...
1) How does increasing the degree of unsaturation affect the rate of the Sn1 and the Sn2 reaction? 2) How does shifting from a cycloalkyl bromide to an aromatic bromide affect the rate of the Sn1 and the Sn2 reaction?
An enzymatic reaction was analyzed for rate versus substrate concentration. Roughly estimate the Vmax and Km...
An enzymatic reaction was analyzed for rate versus substrate concentration. Roughly estimate the Vmax and Km from these measurements. (Hint: Slope = rise/run and remember what Km represents!) vo(nM/s)           [S] (nM) 30                           100,000 29.5                        10,000 29                           1,000 28                           200                         25                           150 20                           100 10                           50 5                              20 a. Vmax = 10 nM/s, Km = 20 nM b. Vmax = 20 nM/s , Km = 50 nM c. Vmax = 40 nM/s , Km = 150 nM d. Vmax =...
The release of product is the rate-limiting for an enzyme. Therefore:         A) A burst of...
The release of product is the rate-limiting for an enzyme. Therefore:         A) A burst of product formation is expected         C) A catalytic triad is used to bind substrate         B) transition state binds weaker than product D) DG°’ for the reaction is positive
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT