27. Explain the reaction that had to occur to detect the free indole (substrate, enzyme, and...

27. Explain the reaction that had to occur to detect the free indole (substrate, enzyme, and products)

Expert Solution

gladiator answered 2 years ago

Sulfanilamide that resembles substrate of an enzyme inhibits the enzyme when added to the reaction mix....

Sulfanilamide that resembles substrate of an enzyme inhibits the enzyme when added to the reaction mix. What type of inhibition is this A) allosteric inhibition B) competitive inhibition C) excitatory allosteric control D) noncompetitive inhibition E) feedback inhibition

An enzyme (select all that apply) 1) is not altered by reaction with a substrate 2)...

An enzyme (select all that apply) 1) is not altered by reaction with a substrate 2) is changed by reaction with a substrateis 3) made of carbohydrate 4) can be reused if not damaged 5) is made of protein 6) slows the rate of chemical reactions 7) increases the rate of chemical reaction Select all that apply. An enzyme 1) is a carbohydrate 2) is a protein 3) is a biological catalyst 4) is changed by reaction with a substrate...

50. The table represents the rates of reaction at specific substrate concentrations for an enzyme that...

50. The table represents the rates of reaction at specific substrate concentrations for an enzyme that displays classical Michaelis-Menten kinetics. Two sets of inhibitor data are also included. Determine the Km and Vmax for the uninhibited enzyme. [s](mM) Without inhibitor Vo (µM/s) With inhibitor A With Inhibitor B 1.3 2.50 1.17 0.62 2.6 4.00 2.10 1.42 6.5 6.30 4.00 2.65 13.0 7.60 5.70 3.12 26.0 9.00 7.20 3.58 Please Please Please....show detailed steps so...

If the substrate concentration is limiting, how does increasing enzyme concentration affect reaction rate? A.Increasing enzyme...

If the substrate concentration is limiting, how does increasing enzyme concentration affect reaction rate? A.Increasing enzyme concentration has no effect on reaction rate. B.Increasing enzyme concentration results in an increase in reaction rate. C.Increasing enzyme concentration decreases reaction rate.

1. (3%) You measure the initial rate of an enzyme reaction as a function of substrate...

1. (3%) You measure the initial rate of an enzyme reaction as a function of substrate concentration in the presence and absence of an inhibitor. The following data are obtained: [S] V0 –Inhibitor +Inhibitor 0.0001 33 17 0.0002 50 29 0.0005 71 50 0.001 83 67 0.002 91 80 0.005 96 91 0.01 98 95 0.02 99 98 0.05 100 99 0.1 100 100 0.2 100 100 a) What is the Vmax in the absence of inhibitor? b) What is...

3. What happens to the enzyme and the substrate during a chemical reaction? What does it...

3. What happens to the enzyme and the substrate during a chemical reaction? What does it mean by enzymes being “specific”? Why is the active site specific for only one type of substrate? Please type the answer. Thanks

2) The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate...

2) The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are given below. [S](mu*M) 5 10 20 50 100 200 500 vo (mu*Mmin-1) 21 62 92 126 185 210 217 a) Estimate Vmax and Km from a direct graph of vo vs [S] (Michaelis-Menton plot). b) Use a Lineweaver-Burk plot to analyze the same data. What are the values of Vmax and Km? c) If the total enzyme concentration is 100 pM,...

What has to occur for an enzyme to catalyze a reaction? Select all correct. A. The...

What has to occur for an enzyme to catalyze a reaction? Select all correct. A. The enzyme and substrate cannot interact with each other B. The enzyme and substrate have to undergo a chemical with each other C. The enzyme and an inhibitor have to come into contact at the active site D. The enzyme needs to become denatured prior to contact E. The enzyme and substrate have to undergo temporary non-covalent interactions with each other F. The enzyme and...

1a) what is the relationship between substrate concentration and reaction rate? b) Describe how the enzyme...

1a) what is the relationship between substrate concentration and reaction rate? b) Describe how the enzyme structure is effected: i) below the optimal temperature ii)above the optimal temperature? c)Why is enzyme activity reduced at pH values above or below the optimum? How does the enzyme structure change at non-optimum pH?

You have added an irreversible inhibitor to a sample of enzyme and substrate. At this point, the reaction has stopped completely?

You have added an irreversible inhibitor to a sample of enzyme and substrate. At this point, the reaction has stopped completely. What can you do to regain the activity of the enzyme? Removing the irreversible inhibitor should get the reaction working again. Adding more substrate will increase the rate of reaction. Adding more inhibitor should get the reaction up to speed again. The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity.

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