You quantify nDNA from a plant and get 145ng/ul from your nanodrop result. Assuming you want...

You quantify nDNA from a plant and get 145ng/ul from your nanodrop result. Assuming you want to add 1500 copies of a chloroplast DNA (cpDNA) locus to a PCR reaction, to what concentration should you dilute the DNA? Assume the genome size is 5 x 1011bp, ploidy is diploid, the chloroplast copy number ratio to nDNA is 50 to 1 , and you’re adding 2uL of diluted DNA.

Expert Solution

Answer: Please check the images. Take 1.4ul of DNA and add 48.6ul of water. This dilution having 750CpDNAs/ul. We required 1500 CpDNA copies for PCR and have to add 2ul. So if add 2ul from above-diluted stock, it will contain 1500 CpDNAs in 2ul.

gladiator answered 2 years ago

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