QUESTION 9 If a restriction enzyme produces blunt ends, how many single stranded nucleotides does it...

QUESTION 9

If a restriction enzyme produces blunt ends, how many single stranded nucleotides does it have hanging off each end of DNA

A.
0

B.
1

C.
2

D.
3

QUESTION 28

Synthetic biology predicts that the minimal set of essential genes is in the tens of thousands.

A. True

B. False

QUESTION 31

Glucose inhibits the activity of what enzyme?

A.
adenylyl cyclase

B.
Beta-galactosidase

C.
cAMP

D.
RNA polymerase

Expert Solution

Ans

1. A is correct

Blunt Ends cut by the restriction endonucleases are a straight cut, down through the DNA that results in a flat pair of bases on the ends of the DNA. So there will be no single stranded nucleotides hanging.

2. False

Essential genes are those genes of an organism that are thought to be critical for its survival. Experiments concluded that the absolutely required number of genes for bacteria is on the order of about 250–300. So it cannot be tens of thousands.

3. Ans is A

A,C. Glucose inhibits the activity of adenylyl cyclase which further inhibits the cAMP. Adenylyl Cyclase is an enzyme that catalyzes the conversion of adenosine triphosphate (ATP) to cyclic adenosine monophosphate (cAMP).THE cAMP is low when flucoseeis present , because of the inhibition of adenylyl cyclase enzyme.

B. Glucode. Inhibits the synthesis of beta galactosidase.

D. Glucose breakdown product inhibits the formation of the CAP-cAMP complex required for RNA polymerase to attach at the lac promoter site.

gladiator answered 2 years ago

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