Question

1. What is the reaction in Glycolysis pathway that generates NADH ?
   (write reaction without structures)

2. Write the name of one key allosteric enzyme in the Glycolysis pathway ?

3. What is the net products of catabolism of one glucose in Glycolysis pathway ?

4. What is Glycogenesis and what is the name of the enzyme that catalyzes it ?

5. What is the effects of Insulin and Glucagon on Glycogenesis ?

Answer 1

The reaction generating NADH in the Glycolysis pathway is reaction no. 6. It will generate two molecules of NADH as Dihydroxyacetone phosphate will also be converted to Glyceraldehyde 3 Phosphate which will convert to 1,3 Bisphosphoglycerate with help of enzyme dehydrogenase and NADH is produced.

The allosteric enzyme in the Glycolysis pathway is Phosphofructokinase which catalyzes the reaction no. 3. This enzyme helps in the regulation of glycolysis. It is inhibite by ATP, Citrate and acidic pH whereas stimulated by AMP, ADP, Fructose 2,6 bisphosphate.. Glycolysis:

Glucose+2 NAD + 2 ADP+ 2 pi------> 2 Pyruvate+ 2 NADH+ 2 H + 2 ATP + 2 H2O.

Glycogenesis is the polymerization of glucose into glycogen. thermodynamically it is an endergonic process and requires ATP and UTP. It occurs mainly in the muscle and the liver.

Glycogen synthase is the key enzyme of the glycogenesis that links two glucose residues on an already existing chain of glucose molecules.

glucagon increases glycogen breakdown whereas insulin enhances glycogenesis.