Enolase is an enzyme that catalyzes one reaction in glycolysis in all organisms that carry out this process. The amino acid sequence of enolase is similar but not identical in the organisms. Researchers purified enolase from  Saccharomyces cerevisiae, a single-celled eukaryotic yeast that grows best at  37°C, and from  Chloroflexus aurantiacus, a bacterium that grows best at the much higher temperature of  55°C. The researchers compared the activity of purified enolase from the two organisms by measuring the rate of...