Question

1. if you think of the extracellular matrix (ECM) as a fiber-reinforced matrix, which of the following are the fiber forming elements? (choose as many as apply)

a. elastin

b. glycoproteins

c. proteoglycans

d. collagen

2.if you had to perform an experiment where you had to evaluate cytotoxicity, which of the following assays would be options to consider. (choose as many as apply)

a. western blot

b. PCR

c. MTS assay

d. RT-PCR

e. MTT assay

f. northern blot

g. southern blot

h. lactate dehydrogenase measurement

Answer 1

1. The cell are composed of 30-35% of the fiber elements,these fibers give the cell structure,strength, and stability,it also protects the cell from any mechanical injury.

a. Elastin: It is a fiber bundle of proteins present in extracellular matrix of the connective tissye.This fibers are elastic in nature,so that it can resume its original shape after stretch or contrcting.So this option is correct.

b. Glycoproteins:It is a type of protein having a carbohydrate group attched to it,they play vital role in immuno system,digestive and in reproduction.So this option is wrong.

c. Proteoglycans:It is a type of protein having one or more sugar molecule called glycosaminoglycan.This helps the tissue of the extracellular matrix ti withstand compressional force through hydration and swelling.They are the anchor cell in the matrix fiber.So this option is wrong.

d. Collagen: Is a structural protein present in the extracelluar matrix.It is a strong insoluble fiber protein.So this option is correct.

The correct answer is- (a) & (d)

2. Cytotoxicity: When any substance or any elemet is toxic to the cell is called Cytotoxicity.For example the chemical of the immuno cell or any venom.

a. Western blot: Western blot is used to detect any specific protein present in the blood.We can perform the western blot to confirm the cytotoxicity test.So this option is correct.

b. PCR: Polymerase Chain Reaction is use to amplify the DNA fragment length,where the fragment size is too small.This option is wrong.

c. MTS Assay: This assay can be use to asses cell proliferation,call viability and the cytotoxicity.So this option is correct.

d. RT-PCR: Real Time PCR can be use to detemine and evaluate any type of infection.So this option is correct.

e. MTT-Assay: The MTT-Assay is the abbreviation form of the name of its dye 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. This assay is use to count the viable cells and evaluate the cytotoxicity,potential medical agent and toxic material.So this option is correct.

f. Northern blot: It is use for the detection os assesment of RNA. After analysing the RNA we can apply it to proliferate and then use for the assesment of cytotoxicity.So this option is correct.

g. Southern blot: It is use for the isolation or detection of DNA. We can perform the southern blot use to uderstand gene arrangement and identify the cytotoxicity.This option is correct.

h. Lactate dehydrogenase measurement: It is a cytosolic enzyme present in many type of cells,and determining the colometric assay we can deteermine the cellular Cytotoxicity.So this option is correct.

The correeect answers are- (a),(c),(d),(e),(f),(g) &(h)