Question

1. PCR can generate over 100 _________________ copies of DNA in hours.
2. ____, _____, _____, and _____ are the nucleotides needed to ______ copies of DNA
3. Why do we need to add primers?
4. What can DNA Polymerase withstand that other enzymes cannot?
5. When do your desired fragments start to appear?
6. On cycle ________________, you have over a billion copies of DNA.

1. What is the purpose of the agarose gel?
2. What makes the DNA move?
3. DNA migrates to the _________________ end of the gel.
4. True or False: Long strands of DNA migrate farther than short strands of DNA.
5. Why do we stain the gel?
6. What is agarose made from?
7. Why do we add buffer into the gel mix?
8. What is the gel comb used for?
9. True or False: buffer keeps the gel from drying out.
10. Why add the loading buffer to the DNA sample?
11. Why do we also run a standard?
12. True or False: the red wire will generate a negative charge.
13. True or False: because DNA is negatively charged, it will migrate to the negative pole.
14. How do we know that the current is running in the gel box?
15. Ethidium bromide fits between the ___________ of the DNA ladder and shows up under ___________________ light.
16. Write down your DNA estimates below.

(largest (1) to smallest (3)) bp= base pair

1 ________________bp

2 ________________bp

3 ________________bp

Answer 1

PCR can generate over 100 __billion__ copies of DNA in hours

adenine (A), thymine (T), guanine (G), and cytosine are the nucleotides needed to _make__ copies of DNA

purpose of PCR primers is to provide a “free” 3'-OH group

DNA polymerase joins end

DNA fragmentation is a biochemical hallmark of apoptosis

On cycle __20000_, you have over a billion copies of DNA

Agarose gel electrophoresis separates DNA fragments according to their size

DNA is negatively charged so DNA will migrate towards the positively charged electrode

DNA migrates to the _____positive___ end of the gel

False

it is make stained to make them visible

Agarose is extracted from certain red seaweed

For electrophoresis

True

DNA loading buffers are used for loading DNA samples onto agarose

Standards is used in the lab to separate charged molecules

False

False

DNA will migrate towards positive electrode

between the nitrogenous bases of DNA and fluoresces under UV light.

1. 501

2.415

3.100