1)Please explain the role of alloimmune antibodies in graft rejection?

2)What is the cause of the tissue injury in DTH reactions?

Which of the following viral types enter animal cells via fusion with the animal cell membrane?

Multiple Choice

• enveloped viruses

• non enveloped viruses

• naked viruses

• All of these choices are correct.

compare and contrast the process of spermatogenis and oogeneis.

(1) (26 pts) In lecture we talked about how demographic forces and natural selection can affect patterns of polymorphism (i.e. genetic variation within species).

(a) Describe 2 patterns in genetic variation data (e.g. polymorphism data) that can be caused by balancing selection.

(b) List three ways a bottleneck can affect genetic variation.

(c) Describe 2 approaches one can use to distinguish bottlenecks from selection scenarios at generating the pattern in the data.

1. Which type of chain growth polymer is obtained in nature? Please explain the biological pathway

2. How can you obtain penicillin? (Hint… please consider bioprocessing-fermentation etc

Using the blanks provided, put the steps of involved in mapping a bacterial chromosome by conjugation in chronological order using the numbers 1-6 in the blanks provided.

a. ___ ___ Cells that mated are checked for transfer of other genes from the Hfr to the F– strain.

b. ___ ___ At regular intervals, conjugation is interrupted by shearing the mating bacteria away from each other.

c. ___ ___ For each time point, cells that mated are selected—for instance, by requiring that a prototrophy or antibiotic resistance from each parent be present in one cell.

d. ___ ___ Select a donor Hfr strain and a recipient F– strain. The two strains must be different in genotype for several marker genes—for example, leu– and leu+, azir and azs.

e. ___ ___ The two strains are mixed together in a nutrient medium to allow conjugation to begin. After a few minutes, the culture is diluted to prevent new conjugations.

f. ___ ___ Order and distance of transferred genes are determined by comparing the time required for them to transfer from the Hfr to the F– strain.

3. Based on the article entitled, Environment Society of Oman’s countrywide awareness efforts
to protect heritage ends, (please click here to view the full text) answer the given questions.

i. Point out the components of environmental education and provide short details for each.

ii. Identify the component of environmental education which is the best approach in solving
environmental problem. Justify your answer.
4. Ref

3. Based on the article entitled, Environment Society of Oman’s countrywide awareness efforts
to protect heritage ends, (please click here to view the full text) answer the given questions.

i. Point out the components of environmental education and provide short details for each.

ii. Identify the component of environmental education which is the best approach in solving
environmental problem. Justify your answer.
4. Ref

When temperatures are increased what generally happens to the rates of photosynthesis and respiration in plant tissue?

In general, how much do respiration rates changes relative to a 10°C rise in temperature?

What is the relationship between leaf maturity and respiration rates?

1. If the first aa of a tripeptide is Met (AUG) followed by Ile (AUC) and Pro (CCU), the order of codons in the mRNA for the tripeptide should read
   1. AUGAUGILECCUUAG        c. AUGILECCUU                           
   2. AUGILECCUUAG                d. AUGILECCUUAGUAG

2. The first base of the codon is U, the number of codons beginning with U is
   1. 4                      b. 8                           c. 12              d. 16

3.    A codon for Ile is AUC, therefore its anticodon is
   1. GAU               b. CUA                  c. UAG           d. UGA

4.   The folding of tRNA has contributions from the
   1. the TYC and the D arms together
   2. the TYC alone
   3. the TYC, anticodon and D arms
   4. the TYC, aminoacyl, anticodon and D arms

5.   Protein synthesis in bacteria is faster than in animal cells because
   1. transport of mRNA is faster through the smaller bacterial nucleus
   2. there are more ribosomes in bacteria than in the animal cells
   3. there are many nuclei in the bacteria making more mRNA
   4. the mRNA of bacteria goes directly to the ribosome from the polymerase

1. The enzyme that unwinds the DNA double helix is called
   1. DNA gyrase                c. helicase
   2. primase                       d. ligase
2. RNA polymerase has
   1. 5’- 3’ exonucleases
   2. 3’- 5’ exonucleases
   3. both exonucleases
   4. no exonucleases

3. In the synthesis of RNA the addition proceeds by    
   1. attack of the 3’OH to the a phosphate of the incoming NTP
   2. attack of the 3’OH to the a phosphate of the incoming dNTP
   3. attack of the 5’OH to the a phosphate of the incoming NTP
   4. attack of the 5’OH to the a phosphate of the incoming dNTP                     

4.    The RNA transcript is identical to the
   1. nontemplate strand of DNA
   2. coding strand of DNA but T is replaced by U
   3. coding strand of DNA but U is replaced by T
   4. template strand

5.   The specific binding sites for RNA polymerase on DNA are called
   1. core subunits              
   2. transcripts
   3. introns
   4. promoters

You want to analyze the potential effects of Cisplatin, a well known chemotherapeutic agent on E-cadherin expression in ASPC1 cells at different concentrations (1, 3 and 10 µM). (15 pts)

a. Name and explain at least 3 techniques you can use for this study in detail.

b. What will be your test conditions for this study?

c. With the below information already given to you, how many microliters of the drug will you add to each condition if you are using a 10 cm dish for cell culture?

- Main stock of Cisplatin is 10 mM

- Test concentrations will be 1, 3 and 10 µM.

After glycolysis has been completed (and before pyruvate is processed) most of the usable energy from the original glucose molecule is contained in ___ molecules.

a. Acetyl-Coa

b. ATP

c. NADH and FADH2

d. CO2

e. pyruvate