Question

1) Matching. Fill in the number matching column A with the column B

_______ adsorption chromatography                          1. Solute equilibrates between mobile phase and surface of stat. phase

_______ Reverse phase                                                        2. Effluent from a chromatographic column

_______ ion-exchange chromatography                              3. Relates resolving power of a column with parameters that cause peak                                                                                      broadening        

_______ molecular exclusion chromatography                      4. The most common type of column used in partition chromatography

_______ affinity chromatograhy                                              5. Different size solutes penetrate voids in stationary phase to different                                                                                                      extents  

_______ van Deemter Equation/Plot                         6.   A measure of efficiency of separation and column performance

_______ Resolution                                                    7. Sample preparation method where stationary phase coated on a needle is exposed to sample for collection of  

                                                                                        analyte prior to analysis

_______ diffusion                                                                     8. Monitoring the “trees instead of the forest” in mass spectra

_______ retention factor                                                    9. Ions in mobile phase are attracted to counterions covalently attached                                                                                                    to stationary phase

_______ elution                                                                         10. Net transport of solute from a region of high concentration to low                                                                                                                               concentration

______SPME                                                           11. solute in moblie phase is attracted to highly specific groups covalently attached to stationary phase

_______ SIM                                                                                 12. Relatively non-polar mobile phase and polar stationary phase

_______ split injection                                                         13. Time component resides in stat. phase compared to mobile phase                

_______ Normal phase                                                         14. Helps avoid peak fronting from overloading columns             

Answer 1

Matching. Fill in the number matching column A with the column B

___4____ adsorption chromatography                       1. Solute equilibrates between mobile phase and surface of stat. phase

___1____ Reverse phase                                            2. Effluent from a chromatographic column

___9____ ion-exchange chromatography                   3. Relates resolving power of a column with parameters that cause peak broadening  

      

___5____ molecular exclusion chromatography         4. The most common type of column used in partition chromatography

___11____ affinity chromatography                              5. Different size solutes penetrate voids in stationary phase to different extents

____3___ van Deemter Equation/Plot                         6.   A measure of efficiency of separation and column performance

____8___ Resolution                             7. Sample preparation method where stationary phase coated on a needle is exposed to sample for collection of analyte prior to analysis

___10____ diffusion                                                      8. Monitoring the “trees instead of the forest” in mass spectra

____6___ retention factor                                            9. Ions in mobile phase are attracted to counter ions covalently attached to stationary phase

____2___ elution                                                       10. Net transport of solute from a region of high concentration to low concentration

___13___SPME                                                           11. solute in mobile phase is attracted to highly specific groups covalently attached to stationary phase

____14___ SIM                                                            12. Relatively non-polar mobile phase and polar stationary phase

___7____ split injection                                               13. Time component resides in stat. phase compared to mobile phase    

            

___12____ Normal phase                                             14. Helps avoid peak fronting from overloading columns